Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization
文献类型：期刊论文
| 作者 | Lu, Jiao1,2; Tang, Jinlei1,2; Liu, Yi1,2; Zhu, Xinna1; Zhang, Tongcun2; Zhang, Xueli1 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 93期号:6页码:2455-2462 |
| 关键词 | Regulatory parts Modulation of gene expression Glucose utilization PTS Escherichia coli |
| 英文摘要 | Phosphoenolpyruvate (PEP) is an important precursor for anaerobic production of succinate and malate. Although inactivating PEP/carbohydrate phosphotransferase systems (PTS) could increase PEP supply, the resulting strain had a low glucose utilization rate. In order to improve anaerobic glucose utilization rate for efficient production of succinate and malate, combinatorial modulation of galactose permease (galP) and glucokinase (glk) gene expression was carried out in chromosome of an Escherichia coli strain with inactivated PTS. Libraries of artificial regulatory parts, including promoter and messenger RNA stabilizing region (mRS), were firstly constructed in front of beta-galactosidase gene (lacZ) in E. coli chromosome through lambda-Red recombination. Most regulatory parts selected from mRS library had constitutive strengths under different cultivation conditions. A convenient one-step recombination method was then used to modulate galP and glk gene expression with different regulatory parts. Glucose utilization rates of strains modulated with either galP or glk all increased, and the rates had a positive relation with expression strength of both genes. Combinatorial modulation had a synergistic effect on glucose utilization rate. The highest rate (1.64 g/L h) was tenfold higher than PTS- strain and 39% higher than the wild-type E. coli. These modulated strains could be used for efficient anaerobic production of succinate and malate. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | ENGINEERED ESCHERICHIA-COLI ; PHOSPHOTRANSFERASE SYSTEM ; METABOLIC EVOLUTION ; CHROMOSOMAL GENES ; SUCCINATE ; TRANSPORT ; STRAINS ; YIELD ; FERMENTATION ; INCREASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301181600021 |
| 公开日期 | 2011-12-28 |
| 源URL | [http://localhost/handle/0/201]  |
| 专题 | 天津工业生物技术研究所_微生物代谢工程 张学礼_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Inst Tianjin Ind Biotechnol, Tianjin, Peoples R China 2.Tianjin Univ Sci & Technol, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Jiao,Tang, Jinlei,Liu, Yi,et al. Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,93(6):2455-2462. |
| APA | Lu, Jiao,Tang, Jinlei,Liu, Yi,Zhu, Xinna,Zhang, Tongcun,&Zhang, Xueli.(2012).Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,93(6),2455-2462. |
| MLA | Lu, Jiao,et al."Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 93.6(2012):2455-2462. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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