How carbonyl reductases control stereoselectivity: Approaching the goal of rational design
文献类型：期刊论文
| 作者 | Zhu, Dunming1; Hua, Ling2 |
| 刊名 | PURE AND APPLIED CHEMISTRY
 |
| 出版日期 | 2010 |
| 卷号 | 82期号:1页码:117-128 |
| 关键词 | carbonyl reductase enzyme-substrate docking ketone reduction site-directed mutagenesis stereoselectivity |
| 英文摘要 | Although "Prelog's rule" and "two hydrophobic binding pockets" model have been used to predict and explain the stercoselectivity of enzymatic ketone reduction, the molecular basis of stereorecognition by carbonyl reductases has not been well understood. The stercoselectivity is not only determined by the structures of enzymes and substrates, but also affected by the reaction conditions such as temperature and reaction medium. Structural analysis coupled with site-directed mutagenesis of stereocomplementary carbonyl reductases readily reveals the key elements of controlling stereoselectivity in these enzymes. In our studies, enzyme-substrate docking and molecular modeling have been engaged to understand the enantioselectivity diversity of the carbonyl reductase from Sporobolomyces salmonicolor (SSCR), and to guide site-saturation mutagenesis for altering the enantioselectivity of this enzyme. These studies provide valuable information for our understanding of how the residues involved in substrate binding affect the orientation of bound substrate, and thus Control the reaction stereoselectivity. The in silico docking-guided semi-rational approach should be a useful methodology for discovery of new carbonyl reductases. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SECONDARY ALCOHOL-DEHYDROGENASE ; ERYTHROMYCIN POLYKETIDE SYNTHASE ; MISCIBLE ORGANIC-SOLVENTS ; 2 TROPINONE REDUCTASES ; ACTIVE-SITE WATER ; THERMOANAEROBACTER-ETHANOLICUS ; SPOROBOLOMYCES-SALMONICOLOR ; DIFFERENT STEREOSPECIFICITIES ; KETOREDUCTASE DOMAINS ; SUBSTRATE-SPECIFICITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274709300011 |
| 公开日期 | 2011-08-01 |
| 源URL | [http://localhost/handle/0/61]  |
| 专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, State Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Genencor Int, China Res Ctr, Shanghai 200335, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Dunming,Hua, Ling. How carbonyl reductases control stereoselectivity: Approaching the goal of rational design[J]. PURE AND APPLIED CHEMISTRY,2010,82(1):117-128. |
| APA | Zhu, Dunming,&Hua, Ling.(2010).How carbonyl reductases control stereoselectivity: Approaching the goal of rational design.PURE AND APPLIED CHEMISTRY,82(1),117-128. |
| MLA | Zhu, Dunming,et al."How carbonyl reductases control stereoselectivity: Approaching the goal of rational design".PURE AND APPLIED CHEMISTRY 82.1(2010):117-128. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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