Enantioselective Reduction of Diaryl Ketones Catalyzed by a Carbonyl Reductase from Sporobolomyces salmonicolor and its Mutant Enzymes
文献类型:期刊论文
作者 | Li, Hongmei2; Zhu, Dunming1,2; Hua, Ling3; Biehl, Edward R.2 |
刊名 | ADVANCED SYNTHESIS & CATALYSIS
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出版日期 | 2009-03-01 |
卷号 | 351期号:4页码:583-588 |
关键词 | bioreduction diaryl ketones enantioselectivity enzyme catalysis oxidoreductase |
英文摘要 | The carbonyl reductase from red yeast Sporobolomyces salmonicolor AKU4429 (SSCR) and its mutant enzymes effectively catalyzed the enantioselective reduction of diaryl ketones to give the corresponding chiral alcohols. Both conversion and enantioselectivity were dependent on the co-solvent in the reaction medium. Diaryl ketones with a para-substituent on one of the phenyl groups were reduced with high enantioselectivity (up to 99% ee), which is difficult to achieve using chemical methods such as chiral borane reduction, asymmetric hydrogenation or hydrosilylation. Mutation of SSCR at Q245 resulted in a higher amount of (S)-enantiomer in the products, and in the case of mutant Q245P with para-substituted diaryl ketones as substrate, this effect was so remarkable that the reduction enantio-preference was switched from (R) to (S). The present study provides valuable information about the catalytic properties of the carbonyl reductase SSCR toward the reduction of diaryl ketones, serving as basis for further engineering of this enzyme to develop efficient biocatalysts for highly enantiospecific reduction of diaryl ketones without high electronic dissymmetry or an ortho-substituent on one of the aryl groups. |
WOS标题词 | Science & Technology ; Physical Sciences |
类目[WOS] | Chemistry, Applied ; Chemistry, Organic |
研究领域[WOS] | Chemistry |
关键词[WOS] | ACTIVE ALPHA-PHENYLPYRIDYLMETHANOLS ; ASYMMETRIC HYDROGENATION ; ARYLBORONIC ACIDS ; CELL-CULTURES ; ARYL TRANSFER ; BAKERS-YEAST ; ALDEHYDES ; DIARYLMETHANOLS ; HYDROSILYLATION ; BENZOPHENONES |
收录类别 | SCI ; CCR |
语种 | 英语 |
WOS记录号 | WOS:000264831500013 |
公开日期 | 2011-08-17 |
源URL | [http://localhost/handle/0/83] ![]() |
专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, State Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.So Methodist Univ, Dept Chem, Dallas, TX 75275 USA 3.China Res Ctr, Danisco Div, Shanghai 200335, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Hongmei,Zhu, Dunming,Hua, Ling,et al. Enantioselective Reduction of Diaryl Ketones Catalyzed by a Carbonyl Reductase from Sporobolomyces salmonicolor and its Mutant Enzymes[J]. ADVANCED SYNTHESIS & CATALYSIS,2009,351(4):583-588. |
APA | Li, Hongmei,Zhu, Dunming,Hua, Ling,&Biehl, Edward R..(2009).Enantioselective Reduction of Diaryl Ketones Catalyzed by a Carbonyl Reductase from Sporobolomyces salmonicolor and its Mutant Enzymes.ADVANCED SYNTHESIS & CATALYSIS,351(4),583-588. |
MLA | Li, Hongmei,et al."Enantioselective Reduction of Diaryl Ketones Catalyzed by a Carbonyl Reductase from Sporobolomyces salmonicolor and its Mutant Enzymes".ADVANCED SYNTHESIS & CATALYSIS 351.4(2009):583-588. |
入库方式: OAI收割
来源:天津工业生物技术研究所
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