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  3. 生物催化与绿色化工 朱敦明
  4. 期刊论文
Enantioselective Reduction of Diaryl Ketones Catalyzed by a Carbonyl Reductase from Sporobolomyces salmonicolor and its Mutant Enzymes

文献类型：期刊论文

作者Li, Hongmei2; Zhu, Dunming1,2; Hua, Ling3; Biehl, Edward R.2
刊名ADVANCED SYNTHESIS & CATALYSIS
出版日期2009-03-01
卷号351期号:4页码:583-588
关键词bioreduction diaryl ketones enantioselectivity enzyme catalysis oxidoreductase
英文摘要The carbonyl reductase from red yeast Sporobolomyces salmonicolor AKU4429 (SSCR) and its mutant enzymes effectively catalyzed the enantioselective reduction of diaryl ketones to give the corresponding chiral alcohols. Both conversion and enantioselectivity were dependent on the co-solvent in the reaction medium. Diaryl ketones with a para-substituent on one of the phenyl groups were reduced with high enantioselectivity (up to 99% ee), which is difficult to achieve using chemical methods such as chiral borane reduction, asymmetric hydrogenation or hydrosilylation. Mutation of SSCR at Q245 resulted in a higher amount of (S)-enantiomer in the products, and in the case of mutant Q245P with para-substituted diaryl ketones as substrate, this effect was so remarkable that the reduction enantio-preference was switched from (R) to (S). The present study provides valuable information about the catalytic properties of the carbonyl reductase SSCR toward the reduction of diaryl ketones, serving as basis for further engineering of this enzyme to develop efficient biocatalysts for highly enantiospecific reduction of diaryl ketones without high electronic dissymmetry or an ortho-substituent on one of the aryl groups.
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Applied ; Chemistry, Organic
研究领域[WOS]Chemistry
关键词[WOS]ACTIVE ALPHA-PHENYLPYRIDYLMETHANOLS ; ASYMMETRIC HYDROGENATION ; ARYLBORONIC ACIDS ; CELL-CULTURES ; ARYL TRANSFER ; BAKERS-YEAST ; ALDEHYDES ; DIARYLMETHANOLS ; HYDROSILYLATION ; BENZOPHENONES
收录类别SCI ; CCR
语种英语
WOS记录号WOS:000264831500013
公开日期2011-08-17
源URL[http://localhost/handle/0/83]  
专题天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文
作者单位1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, State Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
2.So Methodist Univ, Dept Chem, Dallas, TX 75275 USA
3.China Res Ctr, Danisco Div, Shanghai 200335, Peoples R China
推荐引用方式
GB/T 7714		 Li, Hongmei,Zhu, Dunming,Hua, Ling,et al. Enantioselective Reduction of Diaryl Ketones Catalyzed by a Carbonyl Reductase from Sporobolomyces salmonicolor and its Mutant Enzymes[J]. ADVANCED SYNTHESIS & CATALYSIS,2009,351(4):583-588.
APA Li, Hongmei,Zhu, Dunming,Hua, Ling,&Biehl, Edward R..(2009).Enantioselective Reduction of Diaryl Ketones Catalyzed by a Carbonyl Reductase from Sporobolomyces salmonicolor and its Mutant Enzymes.ADVANCED SYNTHESIS & CATALYSIS,351(4),583-588.
MLA Li, Hongmei,et al."Enantioselective Reduction of Diaryl Ketones Catalyzed by a Carbonyl Reductase from Sporobolomyces salmonicolor and its Mutant Enzymes".ADVANCED SYNTHESIS & CATALYSIS 351.4(2009):583-588.

入库方式： OAI收割

来源：天津工业生物技术研究所

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