Spectrophotometric Determination of Cysteine with Gold Nanoparticles Stabilized with Single-stranded Oligonucleotides
文献类型：期刊论文
| 作者 | Wang Y ; Wang J ; Yang F ; Yang XR |
| 刊名 | analytical sciences
 |
| 出版日期 | 2010 |
| 卷号 | 26期号:5页码:545-549 |
| 关键词 | SELECTIVE DETECTION COLORIMETRIC DETECTION LIQUID-CHROMATOGRAPHY AMINO-ACIDS HOMOCYSTEINE ELECTRODES DERIVATIZATION ENSEMBLE |
| ISSN号 | 0910-6340 |
| 通讯作者 | yang xr |
| 中文摘要 | a sensitive and selective spectrophotometric detection method for cysteine has been established in this paper. the assay is based on the displacement of single-stranded oligonucleotide (ssdna) adsorbed on the surface of citrate-capped gold nanoparticles (aunps) by cysteine in 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid buffer solution (ph 7.2) and on the phenomenon of salt-induced aunps aggregation. upon addition of cysteine, the red-to-blue color changes of the ssdna-stabilized aunps associated with aggregation under high-salt conditions were easily observed with the naked eye. the absorption ratio at 520 and 600 nm was herein employed to quantify the aunps aggregation process. the calibration curve showed that the absorption ratio increased linearly over the concentration range of 0.1 - 1.3 mu m with a limit of detection of 100 nm. subsequently, the assay was successfully employed to determine cysteine in artificial and pharmaceutical injection samples. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000277658300004 |
| 公开日期 | 2012-06-06 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43732]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang Y,Wang J,Yang F,et al. Spectrophotometric Determination of Cysteine with Gold Nanoparticles Stabilized with Single-stranded Oligonucleotides[J]. analytical sciences,2010,26(5):545-549. |
| APA | Wang Y,Wang J,Yang F,&Yang XR.(2010).Spectrophotometric Determination of Cysteine with Gold Nanoparticles Stabilized with Single-stranded Oligonucleotides.analytical sciences,26(5),545-549. |
| MLA | Wang Y,et al."Spectrophotometric Determination of Cysteine with Gold Nanoparticles Stabilized with Single-stranded Oligonucleotides".analytical sciences 26.5(2010):545-549. |
入库方式： OAI收割
来源：长春应用化学研究所
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