Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide
文献类型：期刊论文
| 作者 | Wang H ; Zhong CY ; Wu JF ; Huang YB ; Liu CB |
| 刊名 | journal of controlled release
 |
| 出版日期 | 2010 |
| 卷号 | 143期号:1页码:64-70 |
| 关键词 | FREE-RADICAL SCAVENGERS PROTEIN TRANSDUCTION DOMAIN TOPICAL DICLOFENAC LOTION DIMETHYLSULFOXIDE DMSO MOLECULAR-MECHANISM NUCLEAR DELIVERY FUSION PROTEINS GENE DELIVERY PEPTIDES AMYLOIDOSIS |
| ISSN号 | 0168-3659 |
| 通讯作者 | liu cb |
| 中文摘要 | cell penetrating peptides (cpps) are promising tools for transducing presynthesized therapeutic molecules which possess low membrane permeability. the poor efficiency of cellular uptake and unexpected cellular localization are still the main obstacles to the development of drug delivery by using cpps. in this study, we investigated the effect of a penetration enhancer, dimethylsulfoxide (dmso), on the penetrating efficiency of a synthetic tat peptide or the tat fusion protein. fitc-labeled tat and tat-gfp were added to 10% dmso or 100 mu m chloroquine pretreated cells, fluorescence uptake into culturing cells was observed using fluorescence microscopy, facs or quantitatively analyzed by a fluorescence spectrum. 10% dmso treatment markedly increased internalization of tat into cells and appeared in a well-distributed pattern throughout the cytosol and nucleus without membrane perforating or detectable cytotoxicity, the enhancement effect by 10% dmso was reduced by endocytosis inhibitors including ammonium chloride and sodium azide. 10% dmso also enhanced tat-apoptin induced apoptosis of carcinoma cells. these findings implicated that dmso can be a novel delivery enhancer appropriate for cpp penetration. (c) 2009 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000276766600009 |
| 公开日期 | 2012-06-06 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43785]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang H,Zhong CY,Wu JF,et al. Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide[J]. journal of controlled release,2010,143(1):64-70. |
| APA | Wang H,Zhong CY,Wu JF,Huang YB,&Liu CB.(2010).Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide.journal of controlled release,143(1),64-70. |
| MLA | Wang H,et al."Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide".journal of controlled release 143.1(2010):64-70. |
入库方式： OAI收割
来源：长春应用化学研究所
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