Synthesis and characterization of a pH-sensitive shielding system for polycation gene carriers
文献类型：期刊论文
| 作者 | Xia JL ; Chen J ; Tian HY ; Chen XS |
| 刊名 | science china-chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 53期号:3页码:502-507 |
| 关键词 | PEGYLATION DEGREE MOLECULAR-WEIGHT DELIVERY PEI BIOCOMPATIBILITY COPOLYMERS EFFICIENT POLYPLEX VECTORS COMPLEX |
| ISSN号 | 1674-7291 |
| 通讯作者 | chen xs |
| 中文摘要 | to increase the in vivo stability of polycation gene carriers, a ph-sensitive shielding system, gamma-benzyl l-glutamate-co-glutamate acid polymer (pga(60) (60 refers to the molar ratio of glutamate acid in the polymer)), was synthesized and characterized. pga(60) showed ph sensitivity at about ph 6.0. pga(60) shielded the positive charge of dna/pei (1:1) complexes. gel retardation assay showed that no dna-strand exchange with pga(60) occurred after pga(60) was added to dna/pei complexes at different proportions. mtt cytotoxicity tests demonstrated that neither pga(60) nor dna/pei/pga(60) ternary complexes had cytotoxicity at the test concentration. the transfection efficiency was improved when the positive charge was partly shielded by pga(60). because of the charge repulsion between the surface of cells and ternary complex particles, there was almost no transfection efficiency when the zeta potential of ternary complexes turned to negative. because of the suitable ph sensitive range, pga(60) may be a potential shielding system for polycation gene carriers to be used in vivo. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000276582800007 |
| 公开日期 | 2012-06-06 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43797]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xia JL,Chen J,Tian HY,et al. Synthesis and characterization of a pH-sensitive shielding system for polycation gene carriers[J]. science china-chemistry,2010,53(3):502-507. |
| APA | Xia JL,Chen J,Tian HY,&Chen XS.(2010).Synthesis and characterization of a pH-sensitive shielding system for polycation gene carriers.science china-chemistry,53(3),502-507. |
| MLA | Xia JL,et al."Synthesis and characterization of a pH-sensitive shielding system for polycation gene carriers".science china-chemistry 53.3(2010):502-507. |
入库方式： OAI收割
来源：长春应用化学研究所
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