Layer-by-layer electrochemical biosensor with aptamer-appended active polyelectrolyte multilayer for sensitive protein determination
文献类型：期刊论文
| 作者 | Du Y ; Chen CG ; Li BL ; Zhou M ; Wang EK ; Dong SJ |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2010 |
| 卷号 | 25期号:8页码:1902-1907 |
| 关键词 | DNA FILMS ELECTRODES ENZYME APTASENSOR RECEPTORS SELECTION LIGANDS SENSORS INVITRO |
| ISSN号 | 0956-5663 |
| 通讯作者 | wang ek |
| 中文摘要 | herein, we report two simple label-free electrochemical aptasensors for protein detection using layer-by-layer (lbl) self-assembled multilayers with ferrocene-appended poly(ethyleneimine) (fc-pei), carbon nanotubes (cnts) and aptamer. in one sensing strategy, the fc-pei, cnts and dna aptamer are lbl assembled on the electrode surface via electrostatic interaction. in the presence of target, the aptamer on the outermost layer of the lbl self-assembled multilayer would catch the target on the electrode interface, which makes a barrier for electrons and inhibits the electro-transfer, resulting in the decreased dpv signals of fc-pei. using this strategy, a wide detection range (0.3-165 ng ml(-1)) for model target thrombin is obtained, with a low detection limit of 0.14 ng ml(-1). in the similar sensing strategy for detection of lysozyme, a wide detection range (0.2 ng ml(-1) to 1.66 mu g ml(-1)) and a low detection limit (0.17 ng ml(-1)) are obtained. these results prove that the lbl sensing strategies developed possess sensitivity, selectivity, stability and generality. (c) 2010 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000276424200007 |
| 公开日期 | 2012-06-06 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43807]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Du Y,Chen CG,Li BL,et al. Layer-by-layer electrochemical biosensor with aptamer-appended active polyelectrolyte multilayer for sensitive protein determination[J]. biosensors & bioelectronics,2010,25(8):1902-1907. |
| APA | Du Y,Chen CG,Li BL,Zhou M,Wang EK,&Dong SJ.(2010).Layer-by-layer electrochemical biosensor with aptamer-appended active polyelectrolyte multilayer for sensitive protein determination.biosensors & bioelectronics,25(8),1902-1907. |
| MLA | Du Y,et al."Layer-by-layer electrochemical biosensor with aptamer-appended active polyelectrolyte multilayer for sensitive protein determination".biosensors & bioelectronics 25.8(2010):1902-1907. |
入库方式： OAI收割
来源：长春应用化学研究所
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