Colorimetric biosensing of mercury(II) ion using unmodified gold nanoparticle probes and thrombin-binding aptamer
文献类型：期刊论文
| 作者 | Wang Y ; Yang F ; Yang XR |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2010 |
| 卷号 | 25期号:8页码:1994-1998 |
| ISSN号 | 0956-5663 |
| 关键词 | SPECTROPHOTOMETRIC DETERMINATION ATOMIC-ABSORPTION BASE-PAIRS HG-II DNA HYBRIDS SENSOR |
| 通讯作者 | yang xr |
| 中文摘要 | a colorimetric assay for the determination of mercury(ii) (hg2+) in the presence of lead(ii) (pb2+) was demonstrated with unmodified gold nanoparticles (aunps) as probes and 15-met thrombin-binding aptamer (tba, 5'-ggttggtgtggttgg-3') as sensing elements. upon the addition of hg2+ or pb2+, tba consisting of six thymidine units and nine guanosine units interacted specifically with both ions to form a hairpin-like or a quadruplex structure, respectively. as a result, these conformation changes facilitated the salt-induced aunp aggregation. subsequently, to eliminate pb2+ interference in the determination of hg2+, a novel technique by the use of a characteristic wavelength of aggregated aunps instead of the universal masking agent of pb2+ (2,6-pyridinedicarboxylic acid, pdca) was herein proposed. a comparison of the absorption spectra of the aggregated aunps in the presence of hg2+ and pb2+ showed that the characteristic wavelength of the aggregated aunps (800 nm) facilitated the determination of hg2+ in the presence of pb2+. the calibration curve showed that the absorbance value at 800 nm increased linearly over the hg2+ concentration range of 0.39-8.89 mu m with a limit of detection of 200 nm. then, the assay was successfully employed to determine hg2+ in several water samples. (c) 2010 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000276424200022 |
| 公开日期 | 2012-06-06 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43808]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang Y,Yang F,Yang XR. Colorimetric biosensing of mercury(II) ion using unmodified gold nanoparticle probes and thrombin-binding aptamer[J]. biosensors & bioelectronics,2010,25(8):1994-1998. |
| APA | Wang Y,Yang F,&Yang XR.(2010).Colorimetric biosensing of mercury(II) ion using unmodified gold nanoparticle probes and thrombin-binding aptamer.biosensors & bioelectronics,25(8),1994-1998. |
| MLA | Wang Y,et al."Colorimetric biosensing of mercury(II) ion using unmodified gold nanoparticle probes and thrombin-binding aptamer".biosensors & bioelectronics 25.8(2010):1994-1998. |
入库方式： OAI收割
来源：长春应用化学研究所
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