A Quadruplex-Based, Label-Free, and Real-Time Fluorescence Assay for RNase H Activity and Inhibition
文献类型：期刊论文
| 作者 | Hu D ; Pu F ; Huang ZZ ; Ren JS ; Qu XG |
| 刊名 | chemistry-a european journal
 |
| 出版日期 | 2010 |
| 卷号 | 16期号:8页码:2605-2610 |
| ISSN号 | 0947-6539 |
| 关键词 | HIV-1 REVERSE-TRANSCRIPTASE HUMAN TELOMERIC DNA RIBONUCLEASE-H HUMAN-IMMUNODEFICIENCY SELECTIVE-INHIBITION IN-VITRO MECHANISM KINETICS HYBRID RECOGNITION |
| 通讯作者 | ren js |
| 中文摘要 | we demonstrate a unique quadruplex-based fluorescence assay for sensitive, facile, real-time, and label-free detection of rnase h activity and inhibition by using a g-quadruplex formation strategy. in our approach, a rna dna substrate was prepared, with the dna strand designed as a quadruplex-forming oligomer. upon cleavage of the rna strand by rnase h, the released g-rich dna strand folds into a quadruplex in the presence of monovalent ions and interacts with a specific g-quadruplex binder, n-methyl mesoporphyrin ix (nmm); this gives a dramatic increase in fluorescence and serves as a reporter of the reaction. this novel assay is simple in design, fast in operation, and is more convenient and promising than other methods. it takes less than 30 min to finish and the detection limit is much better or at least comparable to previous reports. no sophisticated experimental techniques or chemical modification for either rna or dna are required. the assay can be accomplished by using a common spectrophotometer and obviates possible interference with the kinetic behavior of the catalysts. our approach offers an ideal system for high-throughput screening of enzyme inhibitors and demonstrates that the structure of the g-quadruplex can be used as a functional tool in specific fields in the future. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000275685800033 |
| 公开日期 | 2012-06-07 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43853]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hu D,Pu F,Huang ZZ,et al. A Quadruplex-Based, Label-Free, and Real-Time Fluorescence Assay for RNase H Activity and Inhibition[J]. chemistry-a european journal,2010,16(8):2605-2610. |
| APA | Hu D,Pu F,Huang ZZ,Ren JS,&Qu XG.(2010).A Quadruplex-Based, Label-Free, and Real-Time Fluorescence Assay for RNase H Activity and Inhibition.chemistry-a european journal,16(8),2605-2610. |
| MLA | Hu D,et al."A Quadruplex-Based, Label-Free, and Real-Time Fluorescence Assay for RNase H Activity and Inhibition".chemistry-a european journal 16.8(2010):2605-2610. |
入库方式： OAI收割
来源：长春应用化学研究所
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