Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry
文献类型：SCI/SSCI论文
| 作者 | Hou S. J. ; Zhu J. ; Ding M. Y. ; Lv G. H. |
| 发表日期 | 2008 |
| 关键词 | liquid chromatography-tandem mass spectrometry solid-phase extraction gibberellic acid indole-3-acetic acid abscisic acid plant-extracts arabidopsis-thaliana rapid method phytohormones auxin purification immunoassay metabolites hplc |
| 英文摘要 | A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of three representative phytohormones in plant samples: gibberellic acid (GA(3)). indole-3-acetic acid (IAA) and abscisic acid (ABA). A solid-phase extraction (SPE) pretreatment method was used to concentrate and purify the three phytohormones of different groups from plant samples. The separation was carried out on a C(18) reversed-phase column, using methanol/water containing 0.2% formic acid (50:50, v/v) as the isocratic mobile phase at the flow-rate of 1.0 mL min(-1), and the three phytohormones were eluted within 7 min. A linear ion trap mass spectrometer equipped with electrospray ionization source was operated in negative ion mode. Selective reaction monitoring (SRM) was employed for quantitative measurement. The SRM transitions monitored were as 345 -> 239, 301 for GA(3), 174 -> 130 for IAA and 263 -> 153, 219 for ABA. Good linearities were found within the ranges of 5-200 mu g mL(-1) for IAA and 0.005-10 mu g mL(-1) for ABA and GA(3). Their detection limits based on a signal-to-noise ratio of three were 0.005 mu g mL(-1), 2.2 mu g mL(-1) and 0.003 mu g mL(-1) for GA(3), IAA and ABA, respectively. Good recoveries from 95.5% to 102.4% for the three phytohormones were obtained. The results demonstrated that the SPE-LC-MS/MS method developed is highly effective for analyzing trace amounts of the three phytohormones in plant samples. (C) 2008 Elsevier B.V. All rights reserved. |
| 出处 | Talanta |
| 卷 | 76 |
| 期 | 4 |
| 页 | 798-802 |
| 收录类别 | SCI |
| 语种 | 英语 |
| ISSN号 | 0039-9140 |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/23861]  |
| 专题 | 地理科学与资源研究所_历年回溯文献 |
| 推荐引用方式 GB/T 7714 | Hou S. J.,Zhu J.,Ding M. Y.,et al. Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry. 2008. |
入库方式： OAI收割
来源：地理科学与资源研究所
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