Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex
文献类型：期刊论文
| 作者 | Pu F ; Hu D ; Ren JS ; Wang S ; Qu XG |
| 刊名 | langmuir
 |
| 出版日期 | 2010 |
| 卷号 | 26期号:6页码:4540-4545 |
| 关键词 | TIME FLUORESCENCE ASSAY RESTRICTION ENDONUCLEASES ENZYMATIC CLEAVAGE GOLD-NANOPARTICLE CHEMICAL SENSORS DNA CLEAVAGE SIGNAL AMPLIFICATION ENERGY-TRANSFER POLYELECTROLYTES ACIDS |
| ISSN号 | 0743-7463 |
| 通讯作者 | ren js |
| 中文摘要 | by taking advantage of the large signal amplification through efficient fluorescence resonance energy transfer (fret) from the conjugated polymer to the intercalating dye mediated by dna, a new strategy for nuclease assay has been developed using conjugated polymer and dna/intercalating dye complex. the discrimination of dna before or after digestion by nuclease denotes the universal application of the approach, in which either dsdna or ssdna substrates could be used for detecting nuclease activity. this method can be extended to most nucleases by simply changing the substrate dna. the present method is label-free, rapid,and highly sensitive, with a detection limit much better or at least comparable to previous reports. fit addition, this assay is easy to implement for visual detection with the assistance of a uv transilluminator. we reason that a similar strategy can be adopted for the detection of other analytes. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000275226700111 |
| 公开日期 | 2012-06-07 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43869]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Pu F,Hu D,Ren JS,et al. Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex[J]. langmuir,2010,26(6):4540-4545. |
| APA | Pu F,Hu D,Ren JS,Wang S,&Qu XG.(2010).Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex.langmuir,26(6),4540-4545. |
| MLA | Pu F,et al."Universal Platform for Sensitive and Label-Free Nuclease Assay Based on Conjugated Polymer and DNA/Intercalating Dye Complex".langmuir 26.6(2010):4540-4545. |
入库方式： OAI收割
来源：长春应用化学研究所
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