Solid-State Probe Based Electrochemical Aptasensor for Cocaine: A Potentially Convenient, Sensitive, Repeatable, and Integrated Sensing Platform for Drugs
文献类型：期刊论文
| 作者 | Du Y ; Chen CG ; Yin JY ; Li BL ; Zhou M ; Dong SJ ; Wang EK |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 82期号:4页码:1556-1563 |
| 关键词 | MICROCHIP CAPILLARY-ELECTROPHORESIS APTAMER-BASED SENSORS SINGLE-STRANDED-DNA LABEL-FREE ELECTROCHEMILUMINESCENCE DETECTOR IMPEDANCE SPECTROSCOPY SMALL MOLECULES METHYLENE-BLUE ELECTRODES SURFACES |
| ISSN号 | 0003-2700 |
| 通讯作者 | dong sj |
| 中文摘要 | aptamers, which are artificial oligonucleotides selected in vitro, have been employed to design novel biosensors (i.e., aptasensors). in this work, we first constructed a label-free electrochemical aptasensor introducing a probe immobilization technique by the use of a layer-by-layer (lbl) self-assembled multilayer with ferrocene-appended poly(ethyleneimine) (fc-pei) on an indium tin oxide (ito) array electrode for detection of cocaine. the fc-pei and gold nanoparticles (aunps) were lbl assembled on the electrode surface via electrostatic interaction. then, cocaine aptamer fragments, sh-c2, were covalently labeled onto the outermost aunp layer. when the target cocaine and cocaine aptamer c1 were present simultaneously, the sh-c2 layer hybridized partly with c i to bind the cocaine, which led to a decreased differential pulse voltammetry (dpv) signal of fc-pei. this dpv signal change could be used to sensitively detect cocaine with the lowest detectable concentration down to 0.1 mu m and the detection range up to 38.8 mu m, which falls in the the expected range for medical use of detecting drug abuse involving cocaine. meanwhile, the sensor was specific to cocaine in complex biologic fluids such as human plasma, human saliva, etc. the sensing strategy had general applicability, and the detection of thrombin could also be realized, displayed a low detection limit, and exhibited worthiness to other analytes. the aptasensor based on the array electrode held promising potential for integration of the sensing ability in multianalysis for simultaneous detection. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000274466100055 |
| 公开日期 | 2012-06-07 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43926]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Du Y,Chen CG,Yin JY,et al. Solid-State Probe Based Electrochemical Aptasensor for Cocaine: A Potentially Convenient, Sensitive, Repeatable, and Integrated Sensing Platform for Drugs[J]. analytical chemistry,2010,82(4):1556-1563. |
| APA | Du Y.,Chen CG.,Yin JY.,Li BL.,Zhou M.,...&Wang EK.(2010).Solid-State Probe Based Electrochemical Aptasensor for Cocaine: A Potentially Convenient, Sensitive, Repeatable, and Integrated Sensing Platform for Drugs.analytical chemistry,82(4),1556-1563. |
| MLA | Du Y,et al."Solid-State Probe Based Electrochemical Aptasensor for Cocaine: A Potentially Convenient, Sensitive, Repeatable, and Integrated Sensing Platform for Drugs".analytical chemistry 82.4(2010):1556-1563. |
入库方式： OAI收割
来源：长春应用化学研究所
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