The enhancement of transfection efficiency of cationic liposomes by didodecyldimethylammonium bromide coated gold nanoparticles
文献类型：期刊论文
| 作者 | Li D ; Li GP ; Li PC ; Zhang LX ; Liu ZJ ; Wang J ; Wang EK |
| 刊名 | biomaterials
 |
| 出版日期 | 2010 |
| 卷号 | 31期号:7页码:1850-1857 |
| 关键词 | NONVIRAL GENE DELIVERY MEDIATED TRANSFECTION MAMMALIAN-CELLS IN-VITRO DNA VECTORS THERAPY SURFACE MAGNETOFECTION CHOLESTEROL |
| ISSN号 | 0142-9612 |
| 通讯作者 | wang j |
| 中文摘要 | the development of transfection enhancement of liposomes with attributes of high stability and easy handling in gene therapy is challenging. in this study, we report didodecyldimethylammonium bromide (ddab, a cationic lipid) coated gold nanoparticles (ddab-aunps), which can enhance the transfection efficiency generated by two kinds of commercially available cationic liposomes: lipotap and dotap. it showed that ddab-aunps at the optimal concentrations could produce more than 2 times increase when measuring the number of cells expressed green fluorescent protein and 48-fold increase for luciferase levels after transfection, respectively. the electrophoretic mobility shift assay (emsa) and confocal laser scanning microscopy (clsm) experiments showed that more dna molecules binding to the lipoplexes after adding ddab-aunps. in addition, the flow cytometry (fcm) results indicated that ddab-aunps increased cellular uptake efficiency of dna molecules, which might account for the enhancement of transfection efficiency. it has also been found that the ddab-aunps could decrease the cytotoxicity of liposomes to the cells. (c) 2009 elsevier ltd. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000274354400043 |
| 公开日期 | 2012-06-07 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/43947]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li D,Li GP,Li PC,et al. The enhancement of transfection efficiency of cationic liposomes by didodecyldimethylammonium bromide coated gold nanoparticles[J]. biomaterials,2010,31(7):1850-1857. |
| APA | Li D.,Li GP.,Li PC.,Zhang LX.,Liu ZJ.,...&Wang EK.(2010).The enhancement of transfection efficiency of cationic liposomes by didodecyldimethylammonium bromide coated gold nanoparticles.biomaterials,31(7),1850-1857. |
| MLA | Li D,et al."The enhancement of transfection efficiency of cationic liposomes by didodecyldimethylammonium bromide coated gold nanoparticles".biomaterials 31.7(2010):1850-1857. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。