Effect of pH on the Metabolism of Aconitine under Rat Intestinal Bacteria and Analysis of Metabolites Using HPLC/MS-MS(n) Technique
文献类型：期刊论文
| 作者 | Wang XY ; Pi ZF ; Liu WL ; Zhao YF ; Liu SY |
| 刊名 | chinese journal of chemistry
 |
| 出版日期 | 2010 |
| 卷号 | 28期号:12页码:2494-2500 |
| 关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY TANDEM-MASS-SPECTROMETRY DIESTER-DITERPENOID ALKALOIDS QUANTITATIVE-DETERMINATION CARMICHAELI DEBX TUBER BIOTRANSFORMATION CONSTITUENTS STABILITY |
| ISSN号 | 1001-604x |
| 通讯作者 | liu sy |
| 中文摘要 | a semi-quantitative method of mass spectrometry (ms) has been described for the analysis of metabolites of aconitine by rat intestinal bacteria at different ph. at ph 7.0, the rat intestinal bacteria exhibit optimal activity for the metabolism of aconitine. a high-performance liquid chromatography-electrospray ionization multiple-stage mass spectrometry (hplc/esi-ms(n)) method has been applied to investigate the characteristic product ions of metabolites. then, the logical fragmentation pathways of metabolites have been proposed. by comparing the retention time (t(r)) of hplc and the esi-ms(n) data with the data of standard compounds and reports from literature, ten metabolites have been identified and a distinctive metabolite (15-deoxyaconitine) has been deduced first time. the experimental results demonstrate that hplc/esi-ms(n) is a specific and useful method for the identification of metabolites of aconitine. also, in the present paper, the hplc-ms method was introduced to determine the synthetical metabolite prior to the study of the toxicity by the method of bliss. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| 公开日期 | 2012-06-07 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44007]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang XY,Pi ZF,Liu WL,et al. Effect of pH on the Metabolism of Aconitine under Rat Intestinal Bacteria and Analysis of Metabolites Using HPLC/MS-MS(n) Technique[J]. chinese journal of chemistry,2010,28(12):2494-2500. |
| APA | Wang XY,Pi ZF,Liu WL,Zhao YF,&Liu SY.(2010).Effect of pH on the Metabolism of Aconitine under Rat Intestinal Bacteria and Analysis of Metabolites Using HPLC/MS-MS(n) Technique.chinese journal of chemistry,28(12),2494-2500. |
| MLA | Wang XY,et al."Effect of pH on the Metabolism of Aconitine under Rat Intestinal Bacteria and Analysis of Metabolites Using HPLC/MS-MS(n) Technique".chinese journal of chemistry 28.12(2010):2494-2500. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。