Bifunctional Colorimetric Oligonucleotide Probe Based on a G-Quadruplex DNAzyme Molecular Beacon
文献类型：期刊论文
| 作者 | Zhang LB ; Zhu JB ; Li T ; Wang EK |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2011 |
| 卷号 | 83期号:23页码:8871-8876 |
| 关键词 | PEROXIDASE-ACTIVITY TELOMERASE ACTIVITY CATALYTIC DNA CLEAVING DNA HYBRIDIZATION PROTEINS THROMBIN ACID SELECTION ENZYME |
| ISSN号 | 0003-2700 |
| 通讯作者 | wang ek |
| 中文摘要 | a label-free bifunctional colorimetric oligonucleotide probe for dna and protein detection has been developed on the basis of a novel catalytic molecular beacon consisting of two hairpin structures and a split g-quadruplex dnazyme in the middle. the two loops of this molecular beacon consist of thrombin aptamer sequence and the complementary sequence of target dna, which are utilized to sense single-stranded dna and thrombin. the g-quadruplex dnazyme can effectively catalyze the h(2)o(2)-mediated oxidation of 3,3',5,5'-tetramethylbenzidine sulfate to generate colorimetric signal. upon addition of the target, the dna or protein combines with one loop of the hairpin structures, and meanwhile drives the middle g-quadruplex dnazyme to dissociate. this results in a decrease of catalytic activity, enabling the separate analysis of dna and thrombin. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000297481700009 |
| 公开日期 | 2012-06-07 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44195]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang LB,Zhu JB,Li T,et al. Bifunctional Colorimetric Oligonucleotide Probe Based on a G-Quadruplex DNAzyme Molecular Beacon[J]. analytical chemistry,2011,83(23):8871-8876. |
| APA | Zhang LB,Zhu JB,Li T,&Wang EK.(2011).Bifunctional Colorimetric Oligonucleotide Probe Based on a G-Quadruplex DNAzyme Molecular Beacon.analytical chemistry,83(23),8871-8876. |
| MLA | Zhang LB,et al."Bifunctional Colorimetric Oligonucleotide Probe Based on a G-Quadruplex DNAzyme Molecular Beacon".analytical chemistry 83.23(2011):8871-8876. |
入库方式： OAI收割
来源：长春应用化学研究所
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