Thermophilic esterase from the archaeon Archaeoglobus fulgidus physically immobilized on hydrophobic macroporous resin: A novel biocatalyst for polyester synthesis
文献类型：期刊论文
| 作者 | Li GQ ; Li QS |
| 刊名 | biotechnology and bioprocess engineering
 |
| 出版日期 | 2011 |
| 卷号 | 16期号:6页码:1201-1207 |
| 关键词 | RING-OPENING POLYMERIZATION LIPASE-B ADSORPTION EPSILON-CAPROLACTONE CATALYZED SYNTHESIS |
| ISSN号 | 1226-8372 |
| 通讯作者 | li gq |
| 中文摘要 | this paper reviews the immobilization of a thermophilic esterase, afest from the archaeon archaeoglobus fulgidus, on a hydrophobic macroporous resin and its application in polyester synthesis using the ring-opening polymerization of e >-caprolactone as a model. using the physical adsorption technique, the afest loading concentration after 24 h was 152 mg afest per g of support. particle size and surface morphology of the immobilized enzyme were investigated using laser scattering analysis and scanning electron microscopy. the effects of enzyme concentration, temperature, reaction time and reaction medium on monomer conversion and product molecular weight were systematically investigated. through the optimization of reaction parameters, poly(e >-caprolactone) was obtained at an almost 100% monomer conversion rate and with a low average molecular weight (< 1,100 g/mol). finally, the immobilized enzyme exhibited good operational stability, with a monomer conversion value of more than 55% after four batch reactions. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000297709200019 |
| 公开日期 | 2012-06-07 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44198]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li GQ,Li QS. Thermophilic esterase from the archaeon Archaeoglobus fulgidus physically immobilized on hydrophobic macroporous resin: A novel biocatalyst for polyester synthesis[J]. biotechnology and bioprocess engineering,2011,16(6):1201-1207. |
| APA | Li GQ,&Li QS.(2011).Thermophilic esterase from the archaeon Archaeoglobus fulgidus physically immobilized on hydrophobic macroporous resin: A novel biocatalyst for polyester synthesis.biotechnology and bioprocess engineering,16(6),1201-1207. |
| MLA | Li GQ,et al."Thermophilic esterase from the archaeon Archaeoglobus fulgidus physically immobilized on hydrophobic macroporous resin: A novel biocatalyst for polyester synthesis".biotechnology and bioprocess engineering 16.6(2011):1201-1207. |
入库方式： OAI收割
来源：长春应用化学研究所
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