PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE
文献类型：期刊论文
| 作者 | Wei JZ ; Yang LX ; Hu XL ; Huang YB ; Chen XS ; Jing XB |
| 刊名 | acta polymerica sinica
 |
| 出版日期 | 2011 |
| 期号 | 11页码:1341-1348 |
| 关键词 | REAGENTS 3-TRIFLUOROMETHYL-3-PHENYLDIAZIRINE |
| ISSN号 | 1000-3304 |
| 通讯作者 | wei jz |
| 中文摘要 | a photoaffinity labeling molecule, 4-azidobenzoic acid, was synthesized and introduced into p(la-co-dhp) to form a photoactive copolymer p(la-co-dap). tests showed that proteins could be immobilized on the polymer fibers of p( la-co-dap) within 5 min through a photoaffinity reaction. this suggested potential applications of photoaffinity coupling in protein immobilization and protein detection. furthermore,peg and 4-azidobenzoic acid were reacted in solution under uv irradiation to achieve the modification on peg chains. the peg molecules assembled on gold surface were reacted also with 4-azidobenzoic acid under uv irradiation. bsa adsorption test by ft-spr showed that the 4-azidobenzoic acid moieties were successfully attached to the peg segments so that the protein adsorption ability of the peg surface was changed. in this way, functional groups like -phcooh were introduced onto peg chains in a convenient and quick manner. this would offer a new method to achieve the modification of the polymers lacking of functional groups. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000297825700017 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44216]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wei JZ,Yang LX,Hu XL,et al. PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE[J]. acta polymerica sinica,2011(11):1341-1348. |
| APA | Wei JZ,Yang LX,Hu XL,Huang YB,Chen XS,&Jing XB.(2011).PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE.acta polymerica sinica(11),1341-1348. |
| MLA | Wei JZ,et al."PEG MODIFICATION AND PROTEIN CONJUGATION BY VIRTUE OF 4-AZIDOBENZOIC ACID AS A PHOTOAFFINITY MOLECULE".acta polymerica sinica .11(2011):1341-1348. |
入库方式： OAI收割
来源：长春应用化学研究所
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