Ultrasensitive signal-on DNA biosensor based on nicking endonuclease assisted electrochemistry signal amplification
文献类型：期刊论文
| 作者 | Liu ZY ; Zhang W ; Zhu SY ; Zhang L ; Hu LZ ; Parveen S ; Xu GB |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2011 |
| 卷号 | 29期号:1页码:215-218 |
| 关键词 | SENSOR NANOPARTICLES IMMUNOSENSOR TARGETS PROBE |
| ISSN号 | 0956-5663 |
| 通讯作者 | xu gb |
| 中文摘要 | combining the advantages of signal-on strategy and nicking endonuclease assisted electrochemistry signal amplification (neaesa), a new sensitive and signal-on electrochemical dna biosensor for the sequence specific dna detection based on neaesa has been developed for the first time. a hairpin-shape probe (hp), containing the target dna recognition sequence, is thiol-modified at 5' end and immobilized on gold electrode via au-s bonding. subsequently, the hp modified electrode is hybridized with target dna to form a duplex. then the nicking endonuclease is added and nicks the hp strand in the duplex. after nicking, 3'-ferrocene (fc)-labeled part complementary probe (fc-pcp) is introduced on the electrode surface by hybridizing with the thiol-modified hp fragment, which results in the generation of electrochemical signal. hence, the dna biosensor is constructed successfully. the present dna biosensor shows a wide linear range of 5.0 x 10(-13)-5.0 x 10(-8) m for detecting target dna, with a low detection limit of 0.167 pm. the proposed strategy does not require any amplifying labels (enzymes, dnazymes, nanoparticles, etc.) for biorecognition events, which avoids false-positive results to occur frequently. moreover, the strategy has the benefits of simple preparation, convenient operation, good selectivity, and high sensitivity. with the advantages mentioned above, this simple and sensitive strategy has the potential to be integrated in portable, low cost and simplified devices for diagnostic applications. (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000296174700035 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44222]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu ZY,Zhang W,Zhu SY,et al. Ultrasensitive signal-on DNA biosensor based on nicking endonuclease assisted electrochemistry signal amplification[J]. biosensors & bioelectronics,2011,29(1):215-218. |
| APA | Liu ZY.,Zhang W.,Zhu SY.,Zhang L.,Hu LZ.,...&Xu GB.(2011).Ultrasensitive signal-on DNA biosensor based on nicking endonuclease assisted electrochemistry signal amplification.biosensors & bioelectronics,29(1),215-218. |
| MLA | Liu ZY,et al."Ultrasensitive signal-on DNA biosensor based on nicking endonuclease assisted electrochemistry signal amplification".biosensors & bioelectronics 29.1(2011):215-218. |
入库方式： OAI收割
来源：长春应用化学研究所
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