DNA-Ag nanoclusters as fluorescence probe for turn-on aptamer sensor of small molecules
文献类型：期刊论文
| 作者 | Zhou ZX ; Du Y ; Dong SJ |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2011 |
| 卷号 | 28期号:1页码:33-37 |
| 关键词 | SILVER NANOCLUSTERS SELECTION FLUOROPHORES DISPLACEMENT LIGANDS COCAINE INVITRO |
| ISSN号 | 0956-5663 |
| 通讯作者 | dong sj |
| 中文摘要 | as promising substitutes for organic dyes and quantum dots, few-atom fluorescent silver nanoclusters (ag ncs) have recently gained much attention in a wide range from cellular imaging to chemical/biological detection applications owing to their ultrasmall size (<2 nm), excellent photostability, good biocompatibility and water solubility. herein, we design an aptamer, guanine-rich (g-rich) dna and ag ncs nanocomplex to investigate its ability for the detection of small molecules. the design contains two dna strands which are both chimeric conjugates of the dna aptamer sequence fragment and g-rich sequence fragment. using cocaine as a model molecule, the two dna strands are in free state if there is no cocaine present, and the formed ag ncs through the reduction of ag(+) by nabh(4) show weak fluorescence emission. in the presence of cocaine, however, the two aptamer fragments bind cocaine, which in turn puts the two g-rich sequence fragments in proximity and the fluorescent intensity of dna-ag ncs enhances greatly. as a result, dna-ag ncs are demonstrated as a novel, cost-effective and turn-on fluorescent probe for the analysis of cocaine, with a detection limit of 0.1 mu m. besides, successful detection of adenosine triphosphate (atp) with detection limit of 0.2 mu m demonstrates its potential to be a general method. (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000295661700005 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44282]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhou ZX,Du Y,Dong SJ. DNA-Ag nanoclusters as fluorescence probe for turn-on aptamer sensor of small molecules[J]. biosensors & bioelectronics,2011,28(1):33-37. |
| APA | Zhou ZX,Du Y,&Dong SJ.(2011).DNA-Ag nanoclusters as fluorescence probe for turn-on aptamer sensor of small molecules.biosensors & bioelectronics,28(1),33-37. |
| MLA | Zhou ZX,et al."DNA-Ag nanoclusters as fluorescence probe for turn-on aptamer sensor of small molecules".biosensors & bioelectronics 28.1(2011):33-37. |
入库方式： OAI收割
来源：长春应用化学研究所
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