Highly sensitive and selective detection of thiol-containing biomolecules using DNA-templated silver deposition
文献类型:期刊论文
作者 | Lin YH ; Tao Y ; Ren JS ; Pu F ; Qu XG |
刊名 | biosensors & bioelectronics |
出版日期 | 2011 |
卷号 | 28期号:1页码:339-343 |
ISSN号 | 0956-5663 |
关键词 | GOLD NANOPARTICLES MASS-SPECTROMETRY GLUTATHIONE CYSTEINE DISEASE ASSAY PLASMA METALLIZATION HOMOCYSTEINE AMINOTHIOLS |
通讯作者 | ren js |
中文摘要 | in this work, we report a new fluorescent method for the label-free assay of thiol-containing amino acids and peptide by use of silver deposited dna duplex and the intercalating dye. the sensing approach is based on the specific interactions between thiols and dna-templated silver deposition via robust ag-s bonds. in the presence of thiols, the intercalating dye gives a dramatic increase in fluorescence as a result of the strong interaction between the intercalator and the released dna from silver surfaces. the detection limit of this method is lower than or at least comparable to previous fluorescence-based methods, and the turn-on sensing mode offers additional advantage to efficiently reduce background noise. moreover, the detection and discrimination process can be observed by the naked eye with the aid of an uv transilluminator. this method also exhibits excellent selectivity for these thiol-containing biomolecules over various other amino acids. as far as we know, our method is the first example using the specific interaction between thiols and silver-coated dna to fabricate a turn-on fluorescent sensor for biological thiols with high sensitivity and selectivity. (c) 2011 elsevier b.v. all rights reserved. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000295661700052 |
公开日期 | 2012-06-08 |
源URL | [http://ir.ciac.jl.cn/handle/322003/44283] |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Lin YH,Tao Y,Ren JS,et al. Highly sensitive and selective detection of thiol-containing biomolecules using DNA-templated silver deposition[J]. biosensors & bioelectronics,2011,28(1):339-343. |
APA | Lin YH,Tao Y,Ren JS,Pu F,&Qu XG.(2011).Highly sensitive and selective detection of thiol-containing biomolecules using DNA-templated silver deposition.biosensors & bioelectronics,28(1),339-343. |
MLA | Lin YH,et al."Highly sensitive and selective detection of thiol-containing biomolecules using DNA-templated silver deposition".biosensors & bioelectronics 28.1(2011):339-343. |
入库方式: OAI收割
来源:长春应用化学研究所
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