Solid-State Label-Free Integrated Aptasensor Based on Graphene-Mesoporous Silica-Gold Nanoparticle Hybrids and Silver Microspheres
文献类型：期刊论文
| 作者 | Guo SJ ; Du Y ; Yang X ; Dong SJ ; Wang EK |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2011 |
| 卷号 | 83期号:20页码:8035-8040 |
| 关键词 | ELECTROCHEMICAL APTASENSOR FLUORESCENCE DETECTION COLORIMETRIC DETECTION SENSITIVE DETECTION SMALL MOLECULES FUEL-CELLS APTAMER DNA BIOSENSOR SELECTION |
| ISSN号 | 0003-2700 |
| 通讯作者 | dong sj |
| 中文摘要 | taking advantage of strand-displacement dna polymerization and parallel-motif dna triplex system as dual amplifications, a new electrochemical label-free integrated aptasensor based on silver microspheres (smss) as a separation element and graphene-mesoporous silica-gold nanoparticle (np) hybrids (gsghs) as an enhanced element of the sensing platform was first reported. in this sensing design (schematic representation of the sensing procedure for adenosine triphosphate detection, scheme 1 in manuscript text), which contains an enhanced three-step magnification process, smss with "clean" surface were first used to separate the undesirable aptamer and aptamer-adenosine triphosphate (atp) complex attached on smss surface after aptamer-atp interaction, which lead to the detachment of blocker dna into the solution phase. then, under the assistance of blocker dna, an amplified method based on the inherent signal-transduction mechanism of the hairpin probe and strand-displacement property of dna polymerase was introduced. the obtained duplex dna was used to hybridize with an acceptor dna assembled on electrode to form triplex dna, which could bring a more obvious detection signal compared with the duplex dna without the amplification. the electrochemical signal came from the gsgh-based enhanced sensing interface containing positively charged ferrocene-appended poly(ethyleneimine) (fc-pei). using the above multiple effects, we could achieve the sensitive analysis of a model small molecule-atp (an important "molecular currency" of intracellular energy transfer) in a wide detection range from 0.05 nm to 56.5 nm with the detection limit of 0.023 nm. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000295817500065 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44284]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Guo SJ,Du Y,Yang X,et al. Solid-State Label-Free Integrated Aptasensor Based on Graphene-Mesoporous Silica-Gold Nanoparticle Hybrids and Silver Microspheres[J]. analytical chemistry,2011,83(20):8035-8040. |
| APA | Guo SJ,Du Y,Yang X,Dong SJ,&Wang EK.(2011).Solid-State Label-Free Integrated Aptasensor Based on Graphene-Mesoporous Silica-Gold Nanoparticle Hybrids and Silver Microspheres.analytical chemistry,83(20),8035-8040. |
| MLA | Guo SJ,et al."Solid-State Label-Free Integrated Aptasensor Based on Graphene-Mesoporous Silica-Gold Nanoparticle Hybrids and Silver Microspheres".analytical chemistry 83.20(2011):8035-8040. |
入库方式： OAI收割
来源：长春应用化学研究所
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