RGD targeting hyaluronic acid coating system for PEI-PBLG polycation gene carriers
文献类型：期刊论文
| 作者 | Tian HY ; Lin L ; Chen J ; Chen XS ; Park TG ; Maruyama A |
| 刊名 | journal of controlled release
 |
| 出版日期 | 2011 |
| 卷号 | 155期号:1页码:47-53 |
| 关键词 | SUPPORTED LIPID-BILAYERS INTRACELLULAR DELIVERY HOLE FORMATION DNA COMPLEXES IN-VITRO CELLS POLYMERS POLY(ETHYLENIMINE) POLYETHYLENEIMINE TRANSFECTION |
| ISSN号 | 0168-3659 |
| 通讯作者 | chen xs |
| 中文摘要 | hyaluronic acid (ha), a natural anionic mucopolysaccharide, was used to coat polyethylenimine-poly(gamma-benzyl l-glutamate)/dna (pei-pblg/dna) complexes. ha was further modified by introducing rgd peptide with grafting density of one rgd in every 1.9 ha repeating units. ha can coat the cationic surface of pei-pblg/dna complexes without destroying them even at high weight ratio of ha/pei-pblg/dna = 40/10/1. coating the complexes by ha and ha-rgd caused lower surface charges and little bigger size than the naked pei-pblg/dna ha/pei-pblg/dna has little lower transfection efficiency compared with naked pei-pblg/dna, while the transfection efficiency of ha-rgd/pei-pblg/dna is 9.7 times of ha/pei-pblg/dna for the rgd target bonding affinity to the receptors on the cell surface. ha coating on pei-pblg/dna reduced the electrostatic binding affinity to the cells while the rgd binding affinity for integrin on hela cells can not only compensate the reduced binding affinity but also enhance the affinity for ha-rgd/pei-pblg/dna. rgd and rdg competition assay and lactate dehydrogenase (ldh) release studies further confirmed the specific target functions of rgd on ha. cell viability measurements confirmed the high viability (above 70% viability) of the cells treated with ha-rgd and ha coated complex particles. these results would show that ha-rgd coated pei-pblg/dna complexes have an attractive feature to a targeting in vivo non-viral gene delivery system. (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000295998200009 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44293]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Tian HY,Lin L,Chen J,et al. RGD targeting hyaluronic acid coating system for PEI-PBLG polycation gene carriers[J]. journal of controlled release,2011,155(1):47-53. |
| APA | Tian HY,Lin L,Chen J,Chen XS,Park TG,&Maruyama A.(2011).RGD targeting hyaluronic acid coating system for PEI-PBLG polycation gene carriers.journal of controlled release,155(1),47-53. |
| MLA | Tian HY,et al."RGD targeting hyaluronic acid coating system for PEI-PBLG polycation gene carriers".journal of controlled release 155.1(2011):47-53. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。