Molecular recognition force spectroscopy of a specific lectin-carbohydrate interaction at single-molecule level
文献类型：期刊论文
| 作者 | Li YJ ; Qiao HY ; Xing CY ; Zhang J ; Wang LX ; Wang HD ; Zhang BL ; Tang JL |
| 刊名 | journal of structural biology
 |
| 出版日期 | 2011 |
| 卷号 | 176期号:1页码:46-51 |
| 关键词 | SURFACE-PLASMON RESONANCE S-LAYER NANOARRAYS AFFINITY-TAGS LIVING CELLS MICROSCOPY PROTEIN AFM DNA LOCALIZATION ANTIBODIES |
| ISSN号 | 1047-8477 |
| 通讯作者 | zhang bl |
| 中文摘要 | carbohydrates are involved in many essential biological recognition processes in physiological and pathological states. thus, it is important to understand the mechanism of protein-carbohydrate interactions at molecular level. in the present study, molecular recognition force spectroscopy was applied to investigate the interactions between rca(120), a lectin from ricinus communis, and galactose (gal) and asialofetuin (asf) at the single-molecule level. rca(120) coupled to the afm tip could specifically recognize gal and asf, respectively. the unbinding forces of rca(120)-gal and rca(120)-asf increase linearly with the logarithm of loading rate. the results reveal that the binding capability of rca(120) toward gal is weaker than that of asf, implicating a multivalent effect in the rca(120)-asf interaction. (c) 2011 elsevier inc. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000294885700007 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44328]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li YJ,Qiao HY,Xing CY,et al. Molecular recognition force spectroscopy of a specific lectin-carbohydrate interaction at single-molecule level[J]. journal of structural biology,2011,176(1):46-51. |
| APA | Li YJ.,Qiao HY.,Xing CY.,Zhang J.,Wang LX.,...&Tang JL.(2011).Molecular recognition force spectroscopy of a specific lectin-carbohydrate interaction at single-molecule level.journal of structural biology,176(1),46-51. |
| MLA | Li YJ,et al."Molecular recognition force spectroscopy of a specific lectin-carbohydrate interaction at single-molecule level".journal of structural biology 176.1(2011):46-51. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。