SYNTHESIS AND CHARACTERIZATION OF GENIPIN CROSS-LINKED OLIGOETHYLENIMINE FOR GENE DELIVERY
文献类型：期刊论文
| 作者 | Dong X ; Tian HY ; Chen J ; Xia JL ; Chen XS ; Wei Y |
| 刊名 | acta polymerica sinica
 |
| 出版日期 | 2011 |
| 期号 | 9页码:1086-1091 |
| 关键词 | PEI-PBLG CATIONIC POLYMERS POLYETHYLENIMINE COPOLYMER CARRIER CELLS THERAPY CANCER |
| ISSN号 | 1000-3304 |
| 通讯作者 | dong x |
| 中文摘要 | a novel fluorescent cationic polymer, genipin cross-linked branched oligoethylenimine (1800 da, oei1800), gp, was successfully synthesized in 70% ethanol solution and evaluated as a non-viral gene carrier. the copolymers were characterized by nuclear magnetic resonance spectroscopy ((1)h-nmr) and gel permeation chromatography (gpc). the complexes of copolymers/dna based on gp were measured by dynamic light scattering and zeta potential instrument. the results showed that the synthesized copolymers could efficiently condense dna into nanosized particles (120 similar to 150 nm) with positive surface charges (+ 20 similar to 25 mv). the dna-binding ability of the copolymers was further examined by gel retardation assy. when the mass ratio of polymer and dna was 0.25, the plasmids were fully retarded in 1% agarose gel by gel retardation electrophoresis. moreover, the in vitro cytotoxicity and transfection efficiency of the gp copolymers were respectively tested by the mtt and luciferase assay,using oei1800 and pei25k as the control in the hek293 cells lines. the cell toxicity and gene transfection evaluations showed that the copolymers exhibited lower cytotoxicity and higher gene transfection efficiency than pei25k in the hek293 cell lines. finally, the carriers could be used as the fluorescent labeling materials. therefore, the copolymers may have potential biomedical application as non-viral gene carriers. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000295698100021 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44341]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Dong X,Tian HY,Chen J,et al. SYNTHESIS AND CHARACTERIZATION OF GENIPIN CROSS-LINKED OLIGOETHYLENIMINE FOR GENE DELIVERY[J]. acta polymerica sinica,2011(9):1086-1091. |
| APA | Dong X,Tian HY,Chen J,Xia JL,Chen XS,&Wei Y.(2011).SYNTHESIS AND CHARACTERIZATION OF GENIPIN CROSS-LINKED OLIGOETHYLENIMINE FOR GENE DELIVERY.acta polymerica sinica(9),1086-1091. |
| MLA | Dong X,et al."SYNTHESIS AND CHARACTERIZATION OF GENIPIN CROSS-LINKED OLIGOETHYLENIMINE FOR GENE DELIVERY".acta polymerica sinica .9(2011):1086-1091. |
入库方式： OAI收割
来源：长春应用化学研究所
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