Fluorescence resonance energy transfer dye-labeled probe for fluorescence-enhanced DNA detection: An effective strategy to greatly improve discrimination ability toward single-base mismatch
文献类型：期刊论文
| 作者 | Li HL ; Luo YL ; Sun XP |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2011 |
| 卷号 | 27期号:1页码:167-171 |
| 关键词 | NUCLEIC-ACID DETECTION EFFECTIVE SENSING PLATFORM CARBON NANOTUBES NANOPARTICLES OLIGONUCLEOTIDES HYBRIDIZATION BIOMOLECULES |
| ISSN号 | 0956-5663 |
| 通讯作者 | sun xp |
| 中文摘要 | in this article, we report on the first use of fluorescence resonance energy transfer (fret) dye-labeled probe for fluorescence resonance enhanced dna detection to greatly improve discrimination ability toward single-base mismatch using conjugation polymer poly(p-phenylenediamine) nanobelts (pns) as a sensing platform. the suggested fret dye-labeled probe contains a 5-carboxyfluorescein (fam) group at 5' end of the oligomer as a donor and a 6-carboxy-x-rhodamine (rox) attached to a modified cytosine (c) base as an acceptor, which were separated by three bases. the general concept used in this dna assay is based on adsorption of the fret dye-labeled single-stranded dna (ssdna) probe by pn, which is accompanied by substantial fluorescence quenching and disappearance of fret. the subsequent specific hybridization with its target forms a double-stranded dna (dsdna), resulting in desorption of the hybridized duplex from pn surface accompanied by reoccurrence of fret and fluorescence recovery. it suggests that the discrimination ability of this fret probe based system toward single-base mismatch is about 5.2 times that of the system based on single dye-labeled probe based system. (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000295953300027 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44345]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li HL,Luo YL,Sun XP. Fluorescence resonance energy transfer dye-labeled probe for fluorescence-enhanced DNA detection: An effective strategy to greatly improve discrimination ability toward single-base mismatch[J]. biosensors & bioelectronics,2011,27(1):167-171. |
| APA | Li HL,Luo YL,&Sun XP.(2011).Fluorescence resonance energy transfer dye-labeled probe for fluorescence-enhanced DNA detection: An effective strategy to greatly improve discrimination ability toward single-base mismatch.biosensors & bioelectronics,27(1),167-171. |
| MLA | Li HL,et al."Fluorescence resonance energy transfer dye-labeled probe for fluorescence-enhanced DNA detection: An effective strategy to greatly improve discrimination ability toward single-base mismatch".biosensors & bioelectronics 27.1(2011):167-171. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。