Synthesis and cell-surface binding of lectin-gold nanoparticle conjugates
文献类型：期刊论文
| 作者 | Wang J ; Liu DJ ; Wang ZX |
| 刊名 | analytical methods
 |
| 出版日期 | 2011 |
| 卷号 | 3期号:8页码:1745-1751 |
| 关键词 | CARBOHYDRATE EXPRESSION PROTEIN INTERACTIONS BLOCK-COPOLYMERS RECOGNITION CANCER GLYCOSYLATION NANOSHELLS GLYCOMICS CHEMISTRY BIOLOGY |
| ISSN号 | 1759-9660 |
| 通讯作者 | wang zx |
| 中文摘要 | in this paper, we report a generalized functionalisation strategy, which enables conjugation of lectin to a gold nanoparticle (gnp) surface without affecting the lectin properties. the lectin-gnp conjugates are prepared by coupling lectins to the gnp surfaces through a bifunctional linker peg nhs ester disulfide (nhs-peg-s-s-peg-nhs). subsequently, additional peg-thiol (mpeg-sh) ligand is applied to the gnp suspension in order to block nonspecific adsorption sites on the gnp surface and improve the stability of the lectin-gnp conjugate. the interactions of six lectin-gnp conjugates with six cell lines have been visualized by conventional microscopy and semi-quantitatively analyzed by the uv-visible spectroscopic technique, respectively. the experimental results demonstrate that the lectin-gnp conjugates can bind to living cells with high efficiency and have a great promise to be used as optical probes for monitoring specific affinities of cells with lectins, and evaluating glycosyl complexes on living cell surfaces. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000293515000005 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44435]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang J,Liu DJ,Wang ZX. Synthesis and cell-surface binding of lectin-gold nanoparticle conjugates[J]. analytical methods,2011,3(8):1745-1751. |
| APA | Wang J,Liu DJ,&Wang ZX.(2011).Synthesis and cell-surface binding of lectin-gold nanoparticle conjugates.analytical methods,3(8),1745-1751. |
| MLA | Wang J,et al."Synthesis and cell-surface binding of lectin-gold nanoparticle conjugates".analytical methods 3.8(2011):1745-1751. |
入库方式： OAI收割
来源：长春应用化学研究所
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