Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu
文献类型：期刊论文
| 作者 | Chen RZ ; Liu ZG ; Zhao JM ; Chen RP ; Meng FL ; Zhang M ; Ge WC |
| 刊名 | food chemistry
 |
| 出版日期 | 2011 |
| 卷号 | 127期号:2页码:434-440 |
| 关键词 | IN-VITRO QUANTITATIVE-DETERMINATION PURIFICATION IRRADIATION INHIBITION INJURY MICE RAT |
| ISSN号 | 0308-8146 |
| 通讯作者 | chen rz |
| 中文摘要 | a water-soluble polysaccharide obtained from acanthopanax senticosus leaves (asl), was fractionated by deae-sepharose fast-flow column chromatography, and purified by sephadex g-75 gel-permeation column chromatography. the characteristics of asp-2-1 were determined by chemical analysis, high-performance capillary electrophoresis (hpce), high-performance gel-permeation chromatography (hpgpc). the results show that asp-2-1 contained 89.47% carbohydrate, 7.45% uronic acid, 2.16% protein and seven kinds of monosaccharides including rhamnose, xylose, glucose, mannose, arabinose, galactose and glucuronic acid in a molar ratio of 7.45:18.63:25.15:0.93:8.35:2.79:5.69, with an average molecular weight of about 14,573 da. furthermore, the immunobiological and antioxidant activities, in vitro, of asp-2-1 were evaluated by mit (3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide) assay and ferric-reducing antioxidant power assay (frap), 2,2-dipheny1-1-picrylhydrazyl (dpph(center dot)), superoxide radical ((center dot)o(2)(-)) and hydroxyl radical ((center dot)oh) free radical-scavenging assay, respectively. the results showed that asp-2-1 exhibited significantly higher immunomodulatory activities against the lymphocyte proliferation in vitro, pronounced reductive power (frap value: 785.1 mu m at 0.2 mg/ml), strong hydroxyl radical (89.56% at 1 mg/m1) scavenging activity, moderate superoxide radicals (65.32% at 1 mg/ml) and dpph radicals (68.9% at 1 mg/ml) scavenging activities. asp-2-1 should be explored as a novel and potential natural antioxidant and immunostimulating agent for use in functional foods or medicine. (c) 2011 elsevier ltd. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000288836400008 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44474]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen RZ,Liu ZG,Zhao JM,et al. Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu[J]. food chemistry,2011,127(2):434-440. |
| APA | Chen RZ.,Liu ZG.,Zhao JM.,Chen RP.,Meng FL.,...&Ge WC.(2011).Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu.food chemistry,127(2),434-440. |
| MLA | Chen RZ,et al."Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu".food chemistry 127.2(2011):434-440. |
入库方式： OAI收割
来源：长春应用化学研究所
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