Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin
文献类型：期刊论文
| 作者 | Wang L ; Li CY ; He P ; Fu L ; Zhou YM ; Chen XS |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2011 |
| 卷号 | 32期号:7页码:1622-1628 |
| 关键词 | DRUG-DELIVERY IN-VITRO COMPOSITE SCAFFOLDS VITAMIN-C RELEASE TISSUE RECEPTOR VIVO |
| ISSN号 | 0251-0790 |
| 通讯作者 | zhou ym |
| 中文摘要 | by tripolyphosphate (tpp) as cross-linker, chitosan microspheres (cms) loaded with adrenomedullin (adm) were prepared by an emulsion-ionic cross-linking method. then, porous poly(lactide-co-glycolide) (plga)/nano-hydroxyapatite (nha)/cms scaffold was developed by thermally induced phase separation. scanning electron microscope was used to analyze the characterization of the microspheres and the scaffold and the drug release from them was tested in vitro. also, the hemolytic effect of the scaffold was tested to evaluate the toxicity of the biomaterial. mg63 cell line and huvec cell line were cultured for testing the bioactivity of the scaffold loading adm. after the cells adhered on the scaffold, the cell proliferation and differentiation were analyzed by methyl thiazolyl tetrazolium(mtt) assay, fluorescent staining and alkaline phosphatase(alp) assay. the results show that the diameter of the microspheres is well-distributed and the plga/nha/cms scaffold shows a typical microstructure of polymeric foam. the pores of the scaffold ranged from 30 mu m to 220 mu m and they were interconnected. the hemolytic rate of the biomaterial was less than 5%, which was in line with national standard of medical materials. this study confirmed that plga/nha/cms/adm and plga/nha/cms scaffold could accelerate the proliferation of osteoblast-like cells and vascular endothelial cells and also could stimulate the differentiation of osteoblast-like cells. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 中文 |
| WOS记录号 | WOS:000293303800032 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44484]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang L,Li CY,He P,et al. Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin[J]. chemical journal of chinese universities-chinese,2011,32(7):1622-1628. |
| APA | Wang L,Li CY,He P,Fu L,Zhou YM,&Chen XS.(2011).Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin.chemical journal of chinese universities-chinese,32(7),1622-1628. |
| MLA | Wang L,et al."Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin".chemical journal of chinese universities-chinese 32.7(2011):1622-1628. |
入库方式： OAI收割
来源：长春应用化学研究所
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