Selection, Expression and Purification of Human Abzyme Containing Selenium with Type I Thyroxine Deiodinase Activity
文献类型：期刊论文
| 作者 | Lin F ; Liu LY ; Ni JZ |
| 刊名 | chemical research in chinese universities
 |
| 出版日期 | 2011 |
| 卷号 | 27期号:4页码:619-622 |
| 关键词 | OUTER-RING DEIODINASE HUMAN-ANTIBODIES RAT-LIVER IDENTIFICATION PHAGE LIBRARIES |
| ISSN号 | 1005-9040 |
| 通讯作者 | lin f |
| 中文摘要 | single-chain fragment variable(scfv) antibodies with the substrate binding sites were obtained by repetitive selections from a semi-synthetic phage display antibody library against two haptens-thyroxine(t(4)) and o-methyl-t(4)(o-ch(3)-t(4)). the positive phage clones were determined by elisa and then cloned into vector pet30a(+). the recombined plasmids were identified by dna sequence analysis and transformed into e. coli bl21(de3). the expressed proteins were isolated, purified, identified by western blot analysis, and finally the catalytic group selenocysteine was incorporated into the antibodies binding sites by chemical mutation. the type 1 thyroxine deiodinase activities of the abzymes were 0.006 and 0.008 pg.ml(-1).min(-1), respectively, which were about one tenth that of the mouse intact antibody that was obtained from hybridoma. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000294379100021 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44495]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lin F,Liu LY,Ni JZ. Selection, Expression and Purification of Human Abzyme Containing Selenium with Type I Thyroxine Deiodinase Activity[J]. chemical research in chinese universities,2011,27(4):619-622. |
| APA | Lin F,Liu LY,&Ni JZ.(2011).Selection, Expression and Purification of Human Abzyme Containing Selenium with Type I Thyroxine Deiodinase Activity.chemical research in chinese universities,27(4),619-622. |
| MLA | Lin F,et al."Selection, Expression and Purification of Human Abzyme Containing Selenium with Type I Thyroxine Deiodinase Activity".chemical research in chinese universities 27.4(2011):619-622. |
入库方式： OAI收割
来源：长春应用化学研究所
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