Double-Strand DNA-Templated Formation of Copper Nanoparticles as Fluorescent Probe for Label-Free Aptamer Sensor
文献类型：期刊论文
| 作者 | Zhou ZX ; Du Y ; Dong SJ |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2011 |
| 卷号 | 83期号:13页码:5122-5127 |
| 关键词 | COCAINE MOLECULES SELECTION DISPLACEMENT TRANSDUCTION ADENOSINE LIGANDS COMPLEX INVITRO ATP |
| ISSN号 | 0003-2700 |
| 通讯作者 | dong sj |
| 中文摘要 | double-strand dna (dsdna) can act as an nps)at low concentration of cuso(4), and the formed cu nps efficient template for the formation of copper nanoparticles (cu have excellent fluorescence, whereas a single-strand dna (ssdna) template does not support cu nps' formation. this property of dsdna-cu nps makes it suitable for dna sensing. however, exploration of dsdna-cu nps applied in biological analysis is still at an early stage. in this regard, we report herein for the first time a sensitive, cost-effective, and simple aptamer sensor (aptasensor) using dsdna-cu nps as fluorescent probe. the design consists of a dsdna with reporter dna (here, aptamer) as template for the formation of cu nps, and the formed dsdna-cu nps show high fluorescence. using adenosine triphosphate (atp) as a model analyte, the introduction of atp triggers the structure switching of reporter dna to form aptamer-atp complex, causing the destruction of the double helix and thus no formation of the cu nps, resulting in low fluorescence. the preferable linear range (0.05-500 mu m), sensitivity (lod 28 nm), and simplicity for the detection of atp indicate that dsdna-cu nps may have great prospects in the field of biological analysis. we also use this novel fluorescent probe to determine atp in 1% human serum and human adenocarcinoma hela cells. the dsdna-cu nps probes provide recovery of 104-108% in 1% human serum and a prominent fluorescent signal is obtained in cellular atp assay, revealing the practicality of using dsdna-cu nps for the determination of atp in real samples. besides, this design is simply based on nucleic acid hybridization, so it can be generally applied to other aptamers for label-free detection of a broad range of analytes. successful detection of cocaine with detection limit of 0.1 mu m demonstrates its potential to be a general method. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000292280900016 |
| 公开日期 | 2012-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44523]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhou ZX,Du Y,Dong SJ. Double-Strand DNA-Templated Formation of Copper Nanoparticles as Fluorescent Probe for Label-Free Aptamer Sensor[J]. analytical chemistry,2011,83(13):5122-5127. |
| APA | Zhou ZX,Du Y,&Dong SJ.(2011).Double-Strand DNA-Templated Formation of Copper Nanoparticles as Fluorescent Probe for Label-Free Aptamer Sensor.analytical chemistry,83(13),5122-5127. |
| MLA | Zhou ZX,et al."Double-Strand DNA-Templated Formation of Copper Nanoparticles as Fluorescent Probe for Label-Free Aptamer Sensor".analytical chemistry 83.13(2011):5122-5127. |
入库方式： OAI收割
来源：长春应用化学研究所
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