中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer

文献类型:期刊论文

作者Xi FN ; Gao JQ ; Wang JN ; Wang ZX
刊名journal of electroanalytical chemistry
出版日期2011
卷号656期号:1-2页码:252-257
关键词CELL-SURFACE CARBOHYDRATE CONCANAVALIN-A SACCHAROMYCES-CEREVISIAE GOLD NANOPARTICLES ESCHERICHIA-COLI CARBON NANOTUBES LEUKEMIA-CELLS PERFLUOROCARBON ADSORPTION CAPTURE
ISSN号1572-6657
通讯作者wang zx
中文摘要based on the affinity of lectin with glycosyl complex on cellular surface, a label-free impedimetric biosensor for discrimination and detection of bacteria has been reported. the impedimetric biosensor is fabricated by a simply and reproducibly self-assembled lectin monolayer on the polyelectrolyte modified electrode through electrostatic interaction. two well-known lectins, concanavalin a (con a) and ricinus communis agglutinin (rca), are used to demonstrate proof-of-concept of the recognition interface. the utility of this sensor for selective discrimination of bacterial strain types, e.g., gram-negative bacteria (escherichia coil dh5 alpha and enterobacter cloacae), gram-positive bacterium (bacillus subtilis), fungus (saccharomyces cerevisiae) and mammalian cell (hela cell) with high sensitivity has been achieved. in addition, the con a-bacteria binding process can be inhibited significantly by the saccharide, mannose, giving further proof of the cytosensing mechanism on lectin interface. (c) 2010 elsevier b.v. all rights reserved.
收录类别SCI收录期刊论文
语种英语
WOS记录号WOS:000291768300040
公开日期2012-06-11
源URL[http://ir.ciac.jl.cn/handle/322003/44566]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式
GB/T 7714
Xi FN,Gao JQ,Wang JN,et al. Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer[J]. journal of electroanalytical chemistry,2011,656(1-2):252-257.
APA Xi FN,Gao JQ,Wang JN,&Wang ZX.(2011).Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer.journal of electroanalytical chemistry,656(1-2),252-257.
MLA Xi FN,et al."Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer".journal of electroanalytical chemistry 656.1-2(2011):252-257.

入库方式: OAI收割

来源:长春应用化学研究所

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