Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer
文献类型:期刊论文
作者 | Xi FN ; Gao JQ ; Wang JN ; Wang ZX |
刊名 | journal of electroanalytical chemistry
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出版日期 | 2011 |
卷号 | 656期号:1-2页码:252-257 |
关键词 | CELL-SURFACE CARBOHYDRATE CONCANAVALIN-A SACCHAROMYCES-CEREVISIAE GOLD NANOPARTICLES ESCHERICHIA-COLI CARBON NANOTUBES LEUKEMIA-CELLS PERFLUOROCARBON ADSORPTION CAPTURE |
ISSN号 | 1572-6657 |
通讯作者 | wang zx |
中文摘要 | based on the affinity of lectin with glycosyl complex on cellular surface, a label-free impedimetric biosensor for discrimination and detection of bacteria has been reported. the impedimetric biosensor is fabricated by a simply and reproducibly self-assembled lectin monolayer on the polyelectrolyte modified electrode through electrostatic interaction. two well-known lectins, concanavalin a (con a) and ricinus communis agglutinin (rca), are used to demonstrate proof-of-concept of the recognition interface. the utility of this sensor for selective discrimination of bacterial strain types, e.g., gram-negative bacteria (escherichia coil dh5 alpha and enterobacter cloacae), gram-positive bacterium (bacillus subtilis), fungus (saccharomyces cerevisiae) and mammalian cell (hela cell) with high sensitivity has been achieved. in addition, the con a-bacteria binding process can be inhibited significantly by the saccharide, mannose, giving further proof of the cytosensing mechanism on lectin interface. (c) 2010 elsevier b.v. all rights reserved. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000291768300040 |
公开日期 | 2012-06-11 |
源URL | [http://ir.ciac.jl.cn/handle/322003/44566] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Xi FN,Gao JQ,Wang JN,et al. Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer[J]. journal of electroanalytical chemistry,2011,656(1-2):252-257. |
APA | Xi FN,Gao JQ,Wang JN,&Wang ZX.(2011).Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer.journal of electroanalytical chemistry,656(1-2),252-257. |
MLA | Xi FN,et al."Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer".journal of electroanalytical chemistry 656.1-2(2011):252-257. |
入库方式: OAI收割
来源:长春应用化学研究所
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