CE-electrochemiluminescence with ionic liquid for the facile separation and determination of diester-diterpenoid aconitum alkaloids in traditional Chinese herbal medicine
文献类型：期刊论文
| 作者 | Bao Y ; Yang F ; Yang XR |
| 刊名 | electrophoresis
 |
| 出版日期 | 2011 |
| 卷号 | 32期号:12页码:1515-1521 |
| 关键词 | CAPILLARY-ZONE-ELECTROPHORESIS MASS-SPECTROMETRY CHROMATOGRAPHY URINE CYCLODEXTRIN MESACONITINE EXTRACT PHASE SERUM |
| ISSN号 | 0173-0835 |
| 通讯作者 | yang xr |
| 中文摘要 | a ce-electrochemiluminescence(ce-ecl) detection system, ce/tris(2,2'-bipyridyl) ruthenium(ii)ecl with ionic liquid, was established for the determination of diester-diterpenoid aconitum alkaloids (aconitine (ac), mesaconitine (ma) and hypaconitine (ha)) in traditional chinese herbal medicine. running buffer containing 25 mm borax-20 mm 1-ethyl-3-methylimidazolium tetrafluoroborate at ph 9.15 was used, which resulted in significant changes in separation and obvious enhancement in ecl intensity for ac, ma and ha with similar structures. end-column detection was achieved in 50 mm phosphate buffer with 5 mm ru(bpy)(3)(2+) (ph 9.15) at applied detection voltage of 1.20 v when the distance between the pt working electrode and outlet of capillary (50 cm x 25 mu m id) was set at 150 mu m. one single quantitative analysis of three alkaloids was achieved at a separation voltage of 15 kv within 10 min. moreover, two extraction processes (ethanol extraction and ethyl ether extraction after basification) were investigated. the result showed that ethanol extraction process has higher extraction efficiency than ethyl ether extraction process. under the optimized conditions, the detection limits of ac, ma and ha were 5.62 x 10(-8), 2.78 x 10(-8) and 3.50 x 10(-9) mol/l (s/n = 3), respectively. the method was successfully applied to determine the amounts of ac, ma and ha in the aconitum herbal samples. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000292256100016 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44595]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bao Y,Yang F,Yang XR. CE-electrochemiluminescence with ionic liquid for the facile separation and determination of diester-diterpenoid aconitum alkaloids in traditional Chinese herbal medicine[J]. electrophoresis,2011,32(12):1515-1521. |
| APA | Bao Y,Yang F,&Yang XR.(2011).CE-electrochemiluminescence with ionic liquid for the facile separation and determination of diester-diterpenoid aconitum alkaloids in traditional Chinese herbal medicine.electrophoresis,32(12),1515-1521. |
| MLA | Bao Y,et al."CE-electrochemiluminescence with ionic liquid for the facile separation and determination of diester-diterpenoid aconitum alkaloids in traditional Chinese herbal medicine".electrophoresis 32.12(2011):1515-1521. |
入库方式： OAI收割
来源：长春应用化学研究所
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