Biodegradable mPEG-b-P(MCC-g-OEI) copolymers for efficient gene delivery
文献类型：期刊论文
| 作者 | Dong X ; Tian HY ; Chen L ; Chen J ; Chen XS |
| 刊名 | journal of controlled release
 |
| 出版日期 | 2011 |
| 卷号 | 152期号:1页码:135-142 |
| 关键词 | BLOCK-COPOLYMER PLASMID DNA ADENOASSOCIATED VIRUS NONVIRAL VECTORS IN-VITRO POLYETHYLENIMINE THERAPY TRANSFECTION CARRIER PEI |
| ISSN号 | 0168-3659 |
| 通讯作者 | chen xs |
| 中文摘要 | cationic polymers play an important role in gene delivery. to search for potential non-viral gene carriers, a series of biodegradable cationic polymers, poly(ethylene glycol)-block-poly(carbonates-graft-oligoethylenimine) [mpeg-b-p(mcc-g-oei). ppo] copolymers, were synthesized by grafting different kinds of oligoethylenimine (oei) to the same biodegradable backbone, a derivative of poly(ethylene glycol)-block-polycarbonates. the as-synthesized ppo copolymers were characterized by fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and gel permeation chromatography. two of the as-synthesized ppo copolymers (ppo600 and ppo1800) could efficiently condense dna into nanosized particles (100-140 nm) with positive surface charges when the ppo/dna mass ratio is above 10:1. the cell toxicity and gene transfection evaluations show that ppo copolymers, especially ppo1800, which exhibits lower cytotoxicity and higher gene transfection efficiency than pei25k in the absence and the presence of serum in the cho and cos-7 cell lines, have great potential as non-viral gene carriers. the ppo1800 copolymer images obtained by confocal laser scanning microscopy prove that ppo copolymers could efficiently mediate the entry of plasmid dna into cells. these results show that ppo copolymers may be potential non-viral gene carriers in future applications of gene therapy. (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000292718200017 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44619]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Dong X,Tian HY,Chen L,et al. Biodegradable mPEG-b-P(MCC-g-OEI) copolymers for efficient gene delivery[J]. journal of controlled release,2011,152(1):135-142. |
| APA | Dong X,Tian HY,Chen L,Chen J,&Chen XS.(2011).Biodegradable mPEG-b-P(MCC-g-OEI) copolymers for efficient gene delivery.journal of controlled release,152(1),135-142. |
| MLA | Dong X,et al."Biodegradable mPEG-b-P(MCC-g-OEI) copolymers for efficient gene delivery".journal of controlled release 152.1(2011):135-142. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。