PVP-coated graphene oxide for selective determination of ochratoxin A via quenching fluorescence of free aptamer
文献类型：期刊论文
| 作者 | Sheng LF ; Ren JT ; Miao YQ ; Wang JH ; Wang EK |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2011 |
| 卷号 | 26期号:8页码:3494-3499 |
| 关键词 | BACTERIAL TOXINS IMMUNOSENSOR DIAGNOSTICS MOLECULES SENSORS ARRAYS ASSAY |
| ISSN号 | 0956-5663 |
| 通讯作者 | wang jh |
| 中文摘要 | in this paper, we developed a simple method to detect fungi toxin (ochratoxin a) produced by aspergillus ochraceus and penicillium verrucosumm, utilizing graphene oxide as quencher which can quench the fluorescence of fam (carboxyfluorescein) attached to toxin-specific aptamer. by optimizing the experimental conditions, we obtained the detection limit of our sensing platform based on bare graphene oxide to be 1.9 mu m with a linear detection range from 2 mu m to 35 mu m. selectivity of this sensing platform has been carefully investigated; the results showed that this sensor specifically responded to ochratoxin a without interference from other structure analogues (n-acetyl-l-phenylalanine and warfarin) and with only limited interference from ochratoxin b. experimental data showed that ochratoxin a as well as other structure analogues could adsorb onto the graphene oxide. as compared to the non-protected graphene oxide based biosensor, pvp-protected graphene oxide reveals much lower detection limit (21.8 nm) by two orders of magnitude under the optimized ratio of graphene oxide to pvp concentration. this sensor has also been challenged by testing 1% red wine containing buffer solution spiked with a series of concentration of ochratoxin a. (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000289863900017 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44698]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sheng LF,Ren JT,Miao YQ,et al. PVP-coated graphene oxide for selective determination of ochratoxin A via quenching fluorescence of free aptamer[J]. biosensors & bioelectronics,2011,26(8):3494-3499. |
| APA | Sheng LF,Ren JT,Miao YQ,Wang JH,&Wang EK.(2011).PVP-coated graphene oxide for selective determination of ochratoxin A via quenching fluorescence of free aptamer.biosensors & bioelectronics,26(8),3494-3499. |
| MLA | Sheng LF,et al."PVP-coated graphene oxide for selective determination of ochratoxin A via quenching fluorescence of free aptamer".biosensors & bioelectronics 26.8(2011):3494-3499. |
入库方式： OAI收割
来源：长春应用化学研究所
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