Binding of alpha 1-acid glycoprotein with aconitum alkaloids: an investigation using an intensity fading matrix-assisted laser desorption/ionization Fourier transform mass spectrometry method
文献类型：期刊论文
| 作者 | Liu WL ; Liu S ; Li HL ; Song FR ; Liu ZQ ; Liu SY |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 2011 |
| 卷号 | 25期号:7页码:973-978 |
| 关键词 | EXTRACTIVE ELECTROSPRAY-IONIZATION ANTIBODY-PEPTIDE COMPLEXES ALPHA(1)-ACID GLYCOPROTEIN PROTEIN BINDING RAPID DIFFERENTIATION BIOLOGICAL SAMPLES INHIBITORS AFFINITY DRUGS MS |
| ISSN号 | 0951-4198 |
| 通讯作者 | liu sy |
| 中文摘要 | intensity fading matrix-assisted laser desorption/ionization (if-maldi) mass spectrometry has become an alternative screening approach for the affinity-binding analysis of proteins and peptides with ligands. in this study, an attempt has been made to study the interaction of alpha 1-acid glycoprotein (agp) with aconitum alkaloids by if-maldi fourier transform ion cyclotron resonance mass spectrometry (if-maldi-ft-ms). compared with the nonbinding internal standard, clear reduction in the ion abundances of the target alkaloids was observed with the addition of agp. relative binding affinities of different alkaloids towards the protein could also be estimated using if-maldi-ft-ms. the binding affinity was also investigated by using ultrafiltration liquid chromatography with photodiode array detection coupled to electrospray ionization mass spectrometry (ultrafiltration lc-dad/esi-ms), and results were consistent with that of if-maldi-ft-ms. copyright (c) 2011 john wiley & sons, ltd. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000288461700018 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44732]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu WL,Liu S,Li HL,et al. Binding of alpha 1-acid glycoprotein with aconitum alkaloids: an investigation using an intensity fading matrix-assisted laser desorption/ionization Fourier transform mass spectrometry method[J]. rapid communications in mass spectrometry,2011,25(7):973-978. |
| APA | Liu WL,Liu S,Li HL,Song FR,Liu ZQ,&Liu SY.(2011).Binding of alpha 1-acid glycoprotein with aconitum alkaloids: an investigation using an intensity fading matrix-assisted laser desorption/ionization Fourier transform mass spectrometry method.rapid communications in mass spectrometry,25(7),973-978. |
| MLA | Liu WL,et al."Binding of alpha 1-acid glycoprotein with aconitum alkaloids: an investigation using an intensity fading matrix-assisted laser desorption/ionization Fourier transform mass spectrometry method".rapid communications in mass spectrometry 25.7(2011):973-978. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。