Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR
文献类型：期刊论文
| 作者 | Wei JZ ; Yan LS ; Hu XL ; Chen XS ; Huang YB ; Jing XB |
| 刊名 | colloids and surfaces b-biointerfaces
 |
| 出版日期 | 2011 |
| 卷号 | 83期号:2页码:220-228 |
| 关键词 | SELF-ASSEMBLED MONOLAYERS STERICALLY STABILIZED LIPOSOMES NANOMOLAR DETECTION LIMITS SERUM-PROTEIN ADSORPTION DRUG-DELIVERY QCM-D BIOSENSORS GOLD RESIST SPECTROSCOPY |
| ISSN号 | 0927-7765 |
| 通讯作者 | jing xb |
| 中文摘要 | fourier transform surface plasmon resonance (ft-spr) was utilized to study specific and non-specific interactions between proteins and a biotinylated polymer film by monitoring adsorptions of streptavidin (sav) and bovine serum albumin (bsa) on the polymer films. the biotinylated polymer, poly(lactide-co-2,2-dihydroxymethyl-propylene carbonate-graft-biotin) [p(la-co-dhc/biotin)], was prepared by ring-opening copolymerization of lactide and a oh-bearing cyclic carbonate monomer, followed by biotinylation of the oh groups. the copolymer was coated onto the ft-spr chip and vacuum-dried, hydrated at 70 degrees c, and treated with a blocking agent respectively to achieve different surface status. the ft-spr results showed that the vacuum-dried film had the most bsa adsorption; hydration treatment led to migration of the biotin moieties from inner film to surface and thus resulted in less bsa adsorption; blocking layer on the polymer surface saturated the active sites for physical and chemical adsorptions on the surface and thus weakened the bsa adsorption. adsorption of sav displayed similar polymer-surface-status dependence, i.e., more adsorption on vacuum-dried surface, less adsorption on hydrated surface and the least adsorption on blocked surface. compared with bsa, sav showed more enhanced adsorptions on p(la-co-dhc/biotin) surface because of the specific interaction of biotin moieties in the polymer with sav molecules, especially on the blocked surface. the above semi-quantified results further indicate that the ft-spr system is suitable for investigating interactions between polymer surface and bio-molecules. (c) 2010 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000287268000005 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44746]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wei JZ,Yan LS,Hu XL,et al. Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR[J]. colloids and surfaces b-biointerfaces,2011,83(2):220-228. |
| APA | Wei JZ,Yan LS,Hu XL,Chen XS,Huang YB,&Jing XB.(2011).Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR.colloids and surfaces b-biointerfaces,83(2),220-228. |
| MLA | Wei JZ,et al."Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR".colloids and surfaces b-biointerfaces 83.2(2011):220-228. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。