Label-free detection of nucleic acids by turn-on and turn-off G-quadruplex-mediated fluorescence
文献类型：期刊论文
| 作者 | Ren JT ; Qin HX ; Wang JH ; Luedtke NW ; Wang EK ; Wang J |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2011 |
| 卷号 | 399期号:8页码:2763-2770 |
| 关键词 | C-MYC PROMOTER MOLECULAR BEACONS GOLD NANOPARTICLES DNA DETECTION PROBES TELOMERASE SEQUENCE |
| ISSN号 | 1618-2642 |
| 通讯作者 | wang jh |
| 中文摘要 | in this study we have used two fluorescent probes, tetrakis(diisopropylguanidino)-zinc-phthalocyanine (zn-digp) and n-methylmesoporphyrin ix (nmm), to monitor the reassembly of "split" g-quadruplex probes on hybridization with an arbitrary "target" dna. according to this approach, each split probe is designed to contain half of a g-quadruplex-forming sequence fused to a variable sequence that is complementary to the target dna. upon mixing the individual components, both base-pairing interactions and g-quadruplex fragment reassembly result in a duplex-quadruplex three-way junction that can bind to fluorescent dyes in a g-quadruplex-specific way. the overall fluorescence intensities of the resulting complexes were dependent on the formation of proper base-pairing interactions in the duplex regions, and on the exact identity of the fluorescent probe. compared with samples lacking any "target" dna, the fluorescence intensities of zn-digp-containing samples were lower, and the fluorescence intensities of nmm-containing samples were higher on addition of the target dna. the resulting biosensors based on zn-digp are therefore termed "turn-off" whereas the biosensors containing nmm are defined as "turn-on". both of these biosensors can detect target dnas with a limit of detection in the nanomolar range, and can discriminate mismatched from perfectly matched target dnas. in contrast with previous biosensors based on the peroxidase activity of heme-bound split g-quadruplex probes, the use of fluorescent dyes eliminates the need for unstable sensing components (h(2)o(2), hemin, and abts). our approach is direct, easy to conduct, and fully compatible with the detection of specific dna sequences in biological fluids. having two different types of probe was highly valuable in the context of applied studies, because zn-digp was found to be compatible with samples containing both serum and urine whereas nmm was compatible with urine, but not with serum-containing samples. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000287576700017 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44788]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ren JT,Qin HX,Wang JH,et al. Label-free detection of nucleic acids by turn-on and turn-off G-quadruplex-mediated fluorescence[J]. analytical and bioanalytical chemistry,2011,399(8):2763-2770. |
| APA | Ren JT,Qin HX,Wang JH,Luedtke NW,Wang EK,&Wang J.(2011).Label-free detection of nucleic acids by turn-on and turn-off G-quadruplex-mediated fluorescence.analytical and bioanalytical chemistry,399(8),2763-2770. |
| MLA | Ren JT,et al."Label-free detection of nucleic acids by turn-on and turn-off G-quadruplex-mediated fluorescence".analytical and bioanalytical chemistry 399.8(2011):2763-2770. |
入库方式： OAI收割
来源：长春应用化学研究所
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