Microfluidic Electrochemical Aptameric Assay Integrated On-Chip: A Potentially Convenient Sensing Platform for the Amplified and Multiplex Analysis of Small Molecules
文献类型：期刊论文
| 作者 | Du Y ; Chen CG ; Zhou M ; Dong SJ ; Wang EK |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2011 |
| 卷号 | 83期号:5页码:1523-1529 |
| 关键词 | LABEL-FREE BLOOD-SERUM DNA SENSOR COCAINE BIOSENSOR SELECTION ANALYTES GOLD ATP |
| ISSN号 | 0003-2700 |
| 通讯作者 | dong sj |
| 中文摘要 | aptamers are artificial oligonucleotides that have been widely employed to design biosensors (i.e., aptasensors). in this work, we report a microfluidic electrochemical aptamer-based sensor (mecas) by constructing au-ag dual-metal array three-electrode on-chip for multiplex detection of small molecules. in combination with the microfluidic channels covering on the glass chip, different targets are transported to the au electrodes integrated on different positions of the chip. these electrodes are premodified by different kinds of aptamers, respectively, to fabricate different sensing interfaces which can selectively capture the corresponding target. it is an address-dependent sensing platform; thus, with the use of only one electrochemical probe, multitargets can be recognized and detected according to the readout on a corresponding aptamer-modified electrode. in the sensing strategy, the electrochemical probe, [ru(nh(3))(6)](3+) (ruhex), which can quantitatively bind to surface-confined dna via electrostatic interaction, was used to produce chronocoulometric signal; au nanoparticles (aunps) were used to improve the sensitivity of the sensor by amplifying the detection signals. moreover, the sensing interface fabrication, sample incubation, and electrochemical detection were all performed in microfluidic channels. by using this detection chip, we achieved the multianalysis of two model small molecules, atp, and cocaine, in mixed samples within 40 min. the detection limit of atp was 3 x 10(-10) m, whereas the detection limit of cocaine was 7 x 10(-8) m. this au-ag dual metal electrochemical chip detector integrated mecas was simple, sensitive, and selective. also it is similar to a dosimeter which accumulates signal upon exposure. it held promising potential for designing electrochemical devices with high throughput, high automation, and high integration in multianalysis. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000287685800008 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44794]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Du Y,Chen CG,Zhou M,et al. Microfluidic Electrochemical Aptameric Assay Integrated On-Chip: A Potentially Convenient Sensing Platform for the Amplified and Multiplex Analysis of Small Molecules[J]. analytical chemistry,2011,83(5):1523-1529. |
| APA | Du Y,Chen CG,Zhou M,Dong SJ,&Wang EK.(2011).Microfluidic Electrochemical Aptameric Assay Integrated On-Chip: A Potentially Convenient Sensing Platform for the Amplified and Multiplex Analysis of Small Molecules.analytical chemistry,83(5),1523-1529. |
| MLA | Du Y,et al."Microfluidic Electrochemical Aptameric Assay Integrated On-Chip: A Potentially Convenient Sensing Platform for the Amplified and Multiplex Analysis of Small Molecules".analytical chemistry 83.5(2011):1523-1529. |
入库方式： OAI收割
来源：长春应用化学研究所
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