Label-free detection of polynucleotide single-base mismatch via pyrene probe excimer emission
文献类型：期刊论文
| 作者 | Tang D ; Lu P ; Liao DL ; Yang XY ; Zhang YJ ; Yu C |
| 刊名 | spectrochimica acta part a-molecular and biomolecular spectroscopy
 |
| 出版日期 | 2011 |
| 卷号 | 78期号:2页码:747-752 |
| 关键词 | SOLUBLE CONJUGATED POLYMERS NUCLEIC-ACID PROBES NUCLEOTIDE POLYMORPHISMS OLIGONUCLEOTIDE ARRAYS STRANDED-DNA TAU GENE SNPS FLUORESCENCE MUTATIONS DEMENTIA |
| ISSN号 | 1386-1425 |
| 通讯作者 | zhang yj |
| 中文摘要 | the pyrene probe and pyrene-labeled oligonucleotides (odns) probe are expected to be candidates as fluorescent probe for dna assay. in particular, label-free detection is a very hot because of its simpleness, speediness and cheapness. herein, we have investigated the use of a pyrenylakylammonium salt, a novel fluorescent probe for the detection of one single nucleotide polymorphism (snp) in double stranded dna. after si nuclease digestion, the pyrene probes bind electrostatically to the perfect complement dna and emit a strong excimer emission. however, treatment of the non-complementary dna with s1 nuclease caused nucleotide fragments of less than 5 bases, which could not induce excimer emission. by comparing ratio of excimer to monomer fluorescence between normal and mutant dna after si nuclease digestion. one-base mutation in dna was detected easily. this new method may be applied to the detection of snp. (c) 2010 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000287614500029 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44841]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Tang D,Lu P,Liao DL,et al. Label-free detection of polynucleotide single-base mismatch via pyrene probe excimer emission[J]. spectrochimica acta part a-molecular and biomolecular spectroscopy,2011,78(2):747-752. |
| APA | Tang D,Lu P,Liao DL,Yang XY,Zhang YJ,&Yu C.(2011).Label-free detection of polynucleotide single-base mismatch via pyrene probe excimer emission.spectrochimica acta part a-molecular and biomolecular spectroscopy,78(2),747-752. |
| MLA | Tang D,et al."Label-free detection of polynucleotide single-base mismatch via pyrene probe excimer emission".spectrochimica acta part a-molecular and biomolecular spectroscopy 78.2(2011):747-752. |
入库方式： OAI收割
来源：长春应用化学研究所
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