Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids
文献类型：期刊论文
| 作者 | Han BY ; Wang EK |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2011 |
| 卷号 | 26期号:5页码:2585-2589 |
| 关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY DNA DUPLEXES CYSTEINE HOMOCYSTEINE PROBE FLUOROPHORES CLUSTERS PLASMA THIOLS ASSAY |
| ISSN号 | 0956-5663 |
| 通讯作者 | wang ek |
| 中文摘要 | in this work, we reported a simple and sensitive method to detect biothiols, such as cysteine (cys), homocysteine (hcy) and glutathione (gsh), using fluorescent silver nanoclusters (ag ncs) stabilized by single-stranded dna (dna-ag ncs) as probes. the photoluminescence intensity of dna-ag ncs was found to be quenched effectively with the increase of biothiols concentration due to the formed nonfluorescent coordination complex between dna-ag ncs and biothiols, resulting in the shift-to-red of emission wavelength. but the fluorescence of dna-ag ncs was not changed in the presence of other amino acids at 10-fold higher concentration. satisfactory detection limits and linear relationships of cys, gsh and hcy were obtained, respectively. the resulted plots exhibited good linear relationships in the range from 8.0 x 10(-9) to 1.0 x 10(-7) mol l(-1) (r(2) = 0.984) for cys, 8.0 x 10(-9) to 1.0 x 10(-7) mol l(-1) (r(2) = 0.983) for gsh, and 2.0 x 10(-6) to 6.0 x 10(-7) mol l(-1) (r(2) = 0.999) for hcy, respectively; the detection limits of cys, gsh and hcy were 4.0 nmol l(-1), 4.0 nmol l(-1), and 0.2 mu mol l(-1), respectively. the method was successfully used for the detection of biothiols in human plasma samples. (c) 2010 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000286904400128 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44881]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Han BY,Wang EK. Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids[J]. biosensors & bioelectronics,2011,26(5):2585-2589. |
| APA | Han BY,&Wang EK.(2011).Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids.biosensors & bioelectronics,26(5),2585-2589. |
| MLA | Han BY,et al."Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids".biosensors & bioelectronics 26.5(2011):2585-2589. |
入库方式： OAI收割
来源：长春应用化学研究所
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