Aptamer-based colorimetric biosensing of Ochratoxin A using unmodified gold nanoparticles indicator
文献类型：期刊论文
| 作者 | Yang C ; Wang Y ; Marty JL ; Yang XR |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2011 |
| 卷号 | 26期号:5页码:2724-2727 |
| 关键词 | LINKED-IMMUNOSORBENT-ASSAY SINGLE-STRANDED-DNA MOLECULES SELECTION ENZYME THROMBIN INVITRO LIGANDS PROBES BIND |
| ISSN号 | 0956-5663 |
| 通讯作者 | yang xr |
| 中文摘要 | this work presents an aptasensor for ochratoxin a (ota) using unmodified gold nanoparticles (aunps) indicator. the assay method is based on the conformation change of ota's aptamer in phosphate buffered saline (pbs) containing mg(2+) and ota, and the phenomenon of salt-induced aunps aggregation. a single measurement took only five minutes. circular dichroism spectroscopic experiments revealed for the first time that upon the addition of ota, the conformation of ota's aptamer in pbs buffer changed from random coil structure to compact rigid antiparallel g-quadruplex structure. this compact rigid g-quadruplex structure could not protect aunps against salt-induced aggregation, and thus the color change from red to blue could be observed by the naked eye. the linear range of the colorimetric aptasensor covered a large variation of ota concentration from 20 to 625 nm and the detection limit of 20 nm (3 sigma) was obtained. (c) 2010 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000286904400155 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44882]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang C,Wang Y,Marty JL,et al. Aptamer-based colorimetric biosensing of Ochratoxin A using unmodified gold nanoparticles indicator[J]. biosensors & bioelectronics,2011,26(5):2724-2727. |
| APA | Yang C,Wang Y,Marty JL,&Yang XR.(2011).Aptamer-based colorimetric biosensing of Ochratoxin A using unmodified gold nanoparticles indicator.biosensors & bioelectronics,26(5),2724-2727. |
| MLA | Yang C,et al."Aptamer-based colorimetric biosensing of Ochratoxin A using unmodified gold nanoparticles indicator".biosensors & bioelectronics 26.5(2011):2724-2727. |
入库方式： OAI收割
来源：长春应用化学研究所
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