中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization

文献类型:期刊论文

作者Dong XQ ; Wang Y ; Huang YB ; Liu JQ ; Jing XB
刊名journal of materials chemistry
出版日期2011
卷号21期号:40页码:16147-16152
关键词TISSUE-ENGINEERING APPLICATIONS GLUTATHIONE-S-TRANSFERASE MICROCELLULAR FOAMS MONOLITHS SCAFFOLDS PURIFICATION PERFORMANCE POLYHIPES HYDROGELS POROSITY
ISSN号0959-9428
通讯作者jing xb
中文摘要well-defined porous dextrans bound with glutathione (gsh) were prepared in four steps: (1) vinylation of dextran by reacting it with glycidyl methacrylate (gma), (2) preparation of the aqueous phase with vinylated dextran, surfactant and reduced gsh, (3) hipe polymerization of vinylated dextran initiated by aibn in an oil phase and incorporating gsh onto the dextran chains by chain transfer reactions, and (4) removal of the oil phase and water soluble impurities. these porous materials had interconnected pores, very thin walls and enough gsh moieties for glutathione s-transferase (gst) separation via specific gsh-gst binding. the representative material prepared was filled in the column for separation of the gst from the crude cell lysate. the result shows that this material has a very high selectivity for gst separation.
收录类别SCI收录期刊论文
语种英语
WOS记录号WOS:000295580800042
公开日期2012-06-11
源URL[http://ir.ciac.jl.cn/handle/322003/44937]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式
GB/T 7714
Dong XQ,Wang Y,Huang YB,et al. Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization[J]. journal of materials chemistry,2011,21(40):16147-16152.
APA Dong XQ,Wang Y,Huang YB,Liu JQ,&Jing XB.(2011).Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization.journal of materials chemistry,21(40),16147-16152.
MLA Dong XQ,et al."Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization".journal of materials chemistry 21.40(2011):16147-16152.

入库方式: OAI收割

来源:长春应用化学研究所

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