Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization
文献类型:期刊论文
作者 | Dong XQ ; Wang Y ; Huang YB ; Liu JQ ; Jing XB |
刊名 | journal of materials chemistry
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出版日期 | 2011 |
卷号 | 21期号:40页码:16147-16152 |
关键词 | TISSUE-ENGINEERING APPLICATIONS GLUTATHIONE-S-TRANSFERASE MICROCELLULAR FOAMS MONOLITHS SCAFFOLDS PURIFICATION PERFORMANCE POLYHIPES HYDROGELS POROSITY |
ISSN号 | 0959-9428 |
通讯作者 | jing xb |
中文摘要 | well-defined porous dextrans bound with glutathione (gsh) were prepared in four steps: (1) vinylation of dextran by reacting it with glycidyl methacrylate (gma), (2) preparation of the aqueous phase with vinylated dextran, surfactant and reduced gsh, (3) hipe polymerization of vinylated dextran initiated by aibn in an oil phase and incorporating gsh onto the dextran chains by chain transfer reactions, and (4) removal of the oil phase and water soluble impurities. these porous materials had interconnected pores, very thin walls and enough gsh moieties for glutathione s-transferase (gst) separation via specific gsh-gst binding. the representative material prepared was filled in the column for separation of the gst from the crude cell lysate. the result shows that this material has a very high selectivity for gst separation. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000295580800042 |
公开日期 | 2012-06-11 |
源URL | [http://ir.ciac.jl.cn/handle/322003/44937] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Dong XQ,Wang Y,Huang YB,et al. Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization[J]. journal of materials chemistry,2011,21(40):16147-16152. |
APA | Dong XQ,Wang Y,Huang YB,Liu JQ,&Jing XB.(2011).Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization.journal of materials chemistry,21(40),16147-16152. |
MLA | Dong XQ,et al."Preparation of GSH-functionalized porous dextran for the selective binding of GST by high internal phase emulsion (HIPE) polymerization".journal of materials chemistry 21.40(2011):16147-16152. |
入库方式: OAI收割
来源:长春应用化学研究所
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