Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells
文献类型：期刊论文
| 作者 | Jiang XE ; Musyanovych A ; Rocker C ; Landfester K ; Mailander V ; Nienhaus GU |
| 刊名 | nanoscale
 |
| 出版日期 | 2011 |
| 卷号 | 3期号:5页码:2028-2035 |
| 关键词 | RECEPTOR-MEDIATED ENDOCYTOSIS COATED PIT FORMATION GOLD NANOPARTICLES CELLULAR UPTAKE PROTEIN CORONA HELA-CELLS CLATHRIN PATHWAY SIZE MECHANISM |
| ISSN号 | 2040-3364 |
| 通讯作者 | nienhaus gu |
| 中文摘要 | nanoparticle uptake by living cells is governed by chemical interactions between functional groups on the nanoparticle as well as the receptors on cell surfaces. here we have investigated the uptake of anionic polystyrene (ps) nanoparticles of similar to 100 nm diameter by mesenchymal stem cells (mscs) using spinning-disk confocal optical microscopy combined with a quantitative analysis of the fluorescence images. two types of anionic ps nanoparticles with essentially identical sizes and zeta-potentials were employed in this study, carboxyl-functionalized nanoparticles (cps) and plain ps nanoparticles, both coated with anionic detergent for stabilization. cps nanoparticles were observed to internalize more rapidly and accumulate to a much higher level than plain ps nanoparticles. the relative importance of different uptake mechanisms for the two types of nanoparticles was investigated by using specific inhibitors. cps nanoparticles were internalized mainly via the clathrin-mediated mechanism, whereas plain ps nanoparticles mainly utilized the macropinocytosis pathway. the pronounced difference in the internalization behavior of cps and plain ps nanoparticles points to a specific interaction of the carboxyl group with receptors on the cell surface. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000290531500015 |
| 公开日期 | 2012-06-11 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/44993]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Jiang XE,Musyanovych A,Rocker C,et al. Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells[J]. nanoscale,2011,3(5):2028-2035. |
| APA | Jiang XE,Musyanovych A,Rocker C,Landfester K,Mailander V,&Nienhaus GU.(2011).Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells.nanoscale,3(5),2028-2035. |
| MLA | Jiang XE,et al."Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells".nanoscale 3.5(2011):2028-2035. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。