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Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry

文献类型：EI期刊论文

作者Hou Shengjie ; Zhu Jiang ; Ding Mingyu ; Lv Guohua
发表日期2008
关键词Acetic acid Body fluids Chemicals Chromatographic analysis Chromatography Extraction Fatty acids Fluids High performance liquid chromatography Liquid chromatography Liquid phase epitaxy Mass spectrometers Mass spectrometry Solv
英文摘要A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of three representative phytohormones in plant samples: gibberellic acid (GA3), indole-3-acetic acid (IAA) and abscisic acid (ABA). A solid-phase extraction (SPE) pretreatment method was used to concentrate and purify the three phytohormones of different groups from plant samples. The separation was carried out on a C18 reversed-phase column, using methanol/water containing 0.2% formic acid (50:50, v/v) as the isocratic mobile phase at the flow-rate of 1.0 mL min-1, and the three phytohormones were eluted within 7 min. A linear ion trap mass spectrometer equipped with electrospray ionization source was operated in negative ion mode. Selective reaction monitoring (SRM) was employed for quantitative measurement. The SRM transitions monitored were as 345 &rarr 239, 301 for GA3, 174 &rarr 130 for IAA and 263 &rarr 153, 219 for ABA. Good linearities were found within the ranges of 5-200 μg mL-1 for IAA and 0.005-10 μg mL-1 for ABA and GA3. Their detection limits based on a signal-to-noise ratio of three were 0.005 μg mL-1, 2.2 μg mL-1 and 0.003 μg mL-1 for GA3, IAA and ABA, respectively. Good recoveries from 95.5% to 102.4% for the three phytohormones were obtained. The results demonstrated that the SPE-LC-MS/MS method developed is highly effective for analyzing trace amounts of the three phytohormones in plant samples. © 2008 Elsevier B.V. All rights reserved.
出处Talanta
卷76期:4页:798-802
收录类别EI
语种英语
源URL[http://ir.igsnrr.ac.cn/handle/311030/24875]  
专题地理科学与资源研究所_历年回溯文献
推荐引用方式
GB/T 7714		 Hou Shengjie,Zhu Jiang,Ding Mingyu,et al. Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry. 2008.

入库方式： OAI收割

来源：地理科学与资源研究所

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