An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing
文献类型:期刊论文
作者 | Shi, Chao1,2; Hu, Na1,2; Huang, Hui1; Gao, Ju1; Zhao, You-Jie1; Gao, Li-Zhi1 |
刊名 | PLOS ONE |
出版日期 | 2012-02-22 |
卷号 | 7期号:2页码:e31468 |
ISSN号 | 1932-6203 |
关键词 | iTRAQ technology Neural cell adhesion molecule 1 Neurodegenerative diseases Tianma GASTRODIA-ELATA BLUME PROLYL ISOMERASE PIN1 NEURODEGENERATIVE DISEASES ALZHEIMERS-DISEASE NEUROTROPHIC FACTOR RETINOIC ACID TRIOSEPHOSPHATE ISOMERASE CIS/TRANS ISOMERASES ETHER FRACTION SH-SY5Y CELLS |
通讯作者 | Shi, C (reprint author), Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming, Peoples R China, lgao@mail.kib.ac.cn |
英文摘要 | Background: Chloroplast genomes supply valuable genetic information for evolutionary and functional studies in plants. The past five years have witnessed a dramatic increase in the number of completely sequenced chloroplast genomes with the application of second-generation sequencing technology in plastid genome sequencing projects. However, costeffective high-throughput chloroplast DNA (cpDNA) extraction becomes a major bottleneck restricting the application, as conventional methods are difficult to make a balance between the quality and yield of cpDNAs. |
学科主题 | Biology |
类目[WOS] | Multidisciplinary Sciences |
研究领域[WOS] | Science & Technology - Other Topics |
关键词[WOS] | HIGHER-PLANTS ; ANGIOSPERMS ; RESOLVES ; GENES |
资助信息 | National Science Foundation of China[U0936603]; Natural Science Foundation of Yunnan Province[2008CC016]; Chinese Academy of Sciences (CAS)[KSCX2-YW-N-029]; Talent Program of Yunnan Province[20080A009]; Chinese Department of Science and Technology[2007CB815703]; Kunming Institute of Botany, CAS[672705232515]; Kunming Institute of Botany, CAS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000302875500021 |
公开日期 | 2012-06-07 |
源URL | [http://ir.kib.ac.cn:8080/handle/151853/10541] |
专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Shi, Chao,Hu, Na,Huang, Hui,et al. An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing[J]. PLOS ONE,2012,7(2):e31468. |
APA | Shi, Chao,Hu, Na,Huang, Hui,Gao, Ju,Zhao, You-Jie,&Gao, Li-Zhi.(2012).An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing.PLOS ONE,7(2),e31468. |
MLA | Shi, Chao,et al."An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing".PLOS ONE 7.2(2012):e31468. |
入库方式: OAI收割
来源:昆明植物研究所
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