Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis
文献类型：期刊论文
| 作者 | Xu, Hong1,2,3; Chen, Ling-Jing4; Qu, Li-Jia5; Gu, Hong-Ya5; Li, De-Zhu1,2
|
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 32期号:12页码:1961-1968 |
| 关键词 | Anti-sense Bamboo EMF2 Rapid amplification of cDNA ends Real-time PCR Rescue |
| 英文摘要 | A novel EMBRYONGIC FLOWER2 gene, DlEMF2, was isolated from bamboo, Dendrocalamus latiflorus. It is 2518 bp in length encoding a protein of 629 amino acids. It was expressed in all organs and at a higher level in shoot tissue than in inflorescences. Transgenic Arabidopsis plants expressing antisense DlEMF2 displayed a spectrum of early-flowering phenotypes. Quantitative real-time PCR experiments indicated that the antisense transgene DlEMF2 reduced endogenous AtEMF2 transcript levels leading to the flowering time reduction. Besides, transforming the bamboo DlEMF2 gene into Arabidopsis emf2-1 mutant could rescue phenotype in vegetative and reproductive developmental stages, which reveals that EMF2 has functional conservation between monocots and eudicots. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | POLYCOMB-GROUP PROTEINS ; EXPRESSION |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences [KSCX2-YW-N-029]; National Basic Research Program of China (973 Program) [2007CB411600]; National Natural Science Foundation of China [30770154] |
| 语种 | 英语 |
| WOS记录号 | WOS:000285912900029 |
| 公开日期 | 2012-06-12 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/11195]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 2.Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming 650204, Yunnan, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Univ Calif San Francisco, Dept Hematol Oncol, San Francisco, CA 94143 USA 5.Peking Univ, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Hong,Chen, Ling-Jing,Qu, Li-Jia,et al. Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis[J]. BIOTECHNOLOGY LETTERS,2010,32(12):1961-1968. |
| APA | Xu, Hong,Chen, Ling-Jing,Qu, Li-Jia,Gu, Hong-Ya,&Li, De-Zhu.(2010).Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis.BIOTECHNOLOGY LETTERS,32(12),1961-1968. |
| MLA | Xu, Hong,et al."Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis".BIOTECHNOLOGY LETTERS 32.12(2010):1961-1968. |
入库方式： OAI收割
来源：昆明植物研究所
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