Isolation and ectopic expression of a bamboo MADS-box gene
文献类型：期刊论文
| 作者 | Tian, B; Chen, YY; Yan, YX; Li, DZ
|
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 50期号:3页码:217-224 |
| 关键词 | Dendrocalamus latiflorus MADS-box gene AGL6-like transgenic plants |
| 英文摘要 | A cDNA named DIMADS18 was isolated from the young spikelets of the sweet bamboo, Dendrocalamus latiflorus by RACE. DNA sequence analysis showed that DIMADS18 was composed of full ORF and 3'UTR, but without 5'UTR. The cDNA contained 1039 nucleotides and encoded a putative protein of 249 amino acid residues. The gene displayed the structure of a typical plant MADS box gene, which consisted of an MADS domain, K domain, a short I region, and the C-terminal region. Phylogenetic analysis of plant MADS box genes based on amino acid sequences revealed that DIMADS18 was grouped into the AGAMOUS-LIKE 6 (AGL6)-like subfamily. It was most likely homologous to the OsMADS6 of rice (Oryza sativa), with 88% sequence identity for the entire amino acid sequences. The DIMADS18 also showed relatively high amino acid sequence identity (59%) to AGL6 of Arabidopsis thaliana. To study the functions of DWADS18, DWADS18 cDNA clone driven by the CaMV 35S promoter was transformed into Arabidopsis plants. Transgenic plants of DIMADS18 exhibited the phenotypes of curled leaves, dwarfism, and early flowering with clustered terminal flowers. These results indicated that DIMADS18 may probably be involved in controlling the flowering time of D. latiflorus. |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | FLORAL ORGAN IDENTITY ; FLOWERING TIME ; ARABIDOPSIS-THALIANA ; MOLECULAR-BASIS ; DOMAIN PROTEIN ; FAMILY ; TRANSITION ; PATHWAYS ; EVOLUTION ; PLANTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228412100005 |
| 公开日期 | 2012-06-12 |
| 源URL | [http://ir.kib.ac.cn:8080/handle/151853/11213]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Lsab Plant Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Dalian Univ Technol, Coll Bioengn, Dalian 116622, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, B,Chen, YY,Yan, YX,et al. Isolation and ectopic expression of a bamboo MADS-box gene[J]. CHINESE SCIENCE BULLETIN,2005,50(3):217-224. |
| APA | Tian, B,Chen, YY,Yan, YX,&Li, DZ.(2005).Isolation and ectopic expression of a bamboo MADS-box gene.CHINESE SCIENCE BULLETIN,50(3),217-224. |
| MLA | Tian, B,et al."Isolation and ectopic expression of a bamboo MADS-box gene".CHINESE SCIENCE BULLETIN 50.3(2005):217-224. |
入库方式： OAI收割
来源：昆明植物研究所
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