中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method

文献类型:期刊论文

作者Yang, Jun-Bo1,3; Yang, Jing3; Li, Hong-Tao3; Zhao, Yu2; Yang, Shi-Xiong1
刊名CONSERVATION GENETICS
出版日期2009-10-01
卷号10期号:5页码:1621-1623
关键词Camellia taliensis FIASCO Microsatellite markers Polymorphism Tea plant
英文摘要Camellia taliensis is one of the most important wild tea plants in China, especially in Yunnan Province. In this study, we described the development of 15 microsatellite markers from the genome of C. taliensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 24 samples collected from six wild populations of C. taliensis. The average allele number of the microsatellites was four per locus, ranging from 2 to 7. The observed and expected heterozygosities varied from 0.076 to 0.5833 and from 0.1560 to 0.6917, respectively. Cross-species amplification in other three tea plants showed eleven of them holding promise for sister species. These polymorphic SSR markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy, molecular identification and molecular breeding on this tea plant and its allied species and varieties in section Thea genus Camellia.
学科主题Biodiversity Conservation; Genetics & Heredity
类目[WOS]Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]Biodiversity & Conservation ; Genetics & Heredity
关键词[WOS]SINENSIS ; AMPLIFICATION
收录类别SCI
资助信息National Natural Science Foundation of China [30870169]
语种英语
WOS记录号WOS:000270737400077
公开日期2012-06-12
源URL[http://ir.kib.ac.cn/handle/151853/11215]  
专题昆明植物研究所_中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Jiangxi Agr Univ, Nanchang, Jiangxi, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, SW China Germplasm Bank Wild Species, Kunming 650204, Yunnan, Peoples R China
推荐引用方式
GB/T 7714
Yang, Jun-Bo,Yang, Jing,Li, Hong-Tao,et al. Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method[J]. CONSERVATION GENETICS,2009,10(5):1621-1623.
APA Yang, Jun-Bo,Yang, Jing,Li, Hong-Tao,Zhao, Yu,&Yang, Shi-Xiong.(2009).Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method.CONSERVATION GENETICS,10(5),1621-1623.
MLA Yang, Jun-Bo,et al."Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method".CONSERVATION GENETICS 10.5(2009):1621-1623.

入库方式: OAI收割

来源:昆明植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。