Characterization of 13 microsatellite loci developed from Meconopsis horridula
文献类型：期刊论文
| 作者 | Zhao, Yu1; Zhang, Shi-Bao2 ; Yang, Jing3; Zhang, Lu1
|
| 刊名 | GENETICS AND MOLECULAR BIOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 33期号:3页码:539-541 |
| 关键词 | genetic structure heterozygosity Meconopsis horridula microsatellite markers polymorphism |
| 英文摘要 | Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs. |
| 学科主题 | Biochemistry & Molecular Biology; Genetics & Heredity |
| 类目[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 关键词[WOS] | MARKERS |
| 收录类别 | SCI |
| 资助信息 | Germplasm Bank of Wild Species, China ; Natural Science Foundation of China [30770226]; Natural Science Foundation of Yunnan [2006C0043Q]; West Light Foundation of the Chinese Academy of Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000281913300026 |
| 公开日期 | 2012-06-12 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/11223]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 作者单位 | 1.Jiangxi Agr Univ, Coll Landscape & Art, Nanchang 330045, Jiangxi, Peoples R China 2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Kunming, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yu,Zhang, Shi-Bao,Yang, Jing,et al. Characterization of 13 microsatellite loci developed from Meconopsis horridula[J]. GENETICS AND MOLECULAR BIOLOGY,2010,33(3):539-541. |
| APA | Zhao, Yu,Zhang, Shi-Bao,Yang, Jing,&Zhang, Lu.(2010).Characterization of 13 microsatellite loci developed from Meconopsis horridula.GENETICS AND MOLECULAR BIOLOGY,33(3),539-541. |
| MLA | Zhao, Yu,et al."Characterization of 13 microsatellite loci developed from Meconopsis horridula".GENETICS AND MOLECULAR BIOLOGY 33.3(2010):539-541. |
入库方式： OAI收割
来源：昆明植物研究所
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