Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine
文献类型：期刊论文
| 作者 | Zhang, Weiwei1,2,3; Xu, Dongxue4,5; Niu, Zongliang1,2,3; Yin, Kun1,2,3,5; Liu, Ping1,2,3; Chen, Lingxin1,2,3
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| 刊名 | BIODEGRADATION
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| 出版日期 | 2012-06-01 |
| 卷号 | 23期号:3页码:431-439 |
| 关键词 | Sulfadoxine Pseudomonas sp. Degradation Cytotoxicity |
| ISSN号 | 0923-9820 |
| 产权排序 | [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China; [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Xu, Dongxue] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Xu, Dongxue; Yin, Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
| 中文摘要 | Given that the intensive application of sulfonamides in aquaculture, animal husbandry and malaria treatment has lead to an increase in sulfonamide discharge into the environment, there is an increasing need to find a way to remediate sulfonamide-contaminated sites. The bacterial strain DX7 was isolated from a marine environment and is capable of degrading sulfadoxine. DX7 was identified as a Pseudomonas sp. based on 16S rRNA gene sequencing. Approximately 30% of sulfadoxine was degraded after Pseudomonas sp. DX7 was inoculated into mineral salt plus tryptone media containing 10 mg l(-1) sulfadoxine for 2 days. The degradation efficiency under different environmental conditions was characterized using HPLC. The optimal temperature and pH for sulfadoxine biodegradation were around 30A degrees C and 6.0, respectively. The optimal concentrations of sulfadoxine and tryptone for sulfadoxine biodegradation were determined to be approximately 30 mg l(-1) and between 2.0 and 8.0 g l(-1), respectively. Cytotoxicity analysis indicated that the metabolites of sulfadoxine generated by Pseudomonas sp. DX7 showed significantly reduced cytotoxicity to Hela cells. These results suggest that Pseudomonas sp. DX7 is a new bacterial resource for degrading sulfadoxine and indicate the potential of the isolated strain in the bioremediation of sulfadoxine-contaminated environments. |
| 英文摘要 | Given that the intensive application of sulfonamides in aquaculture, animal husbandry and malaria treatment has lead to an increase in sulfonamide discharge into the environment, there is an increasing need to find a way to remediate sulfonamide-contaminated sites. The bacterial strain DX7 was isolated from a marine environment and is capable of degrading sulfadoxine. DX7 was identified as a Pseudomonas sp. based on 16S rRNA gene sequencing. Approximately 30% of sulfadoxine was degraded after Pseudomonas sp. DX7 was inoculated into mineral salt plus tryptone media containing 10 mg l(-1) sulfadoxine for 2 days. The degradation efficiency under different environmental conditions was characterized using HPLC. The optimal temperature and pH for sulfadoxine biodegradation were around 30A degrees C and 6.0, respectively. The optimal concentrations of sulfadoxine and tryptone for sulfadoxine biodegradation were determined to be approximately 30 mg l(-1) and between 2.0 and 8.0 g l(-1), respectively. Cytotoxicity analysis indicated that the metabolites of sulfadoxine generated by Pseudomonas sp. DX7 showed significantly reduced cytotoxicity to Hela cells. These results suggest that Pseudomonas sp. DX7 is a new bacterial resource for degrading sulfadoxine and indicate the potential of the isolated strain in the bioremediation of sulfadoxine-contaminated environments. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | TANDEM MASS-SPECTROMETRY ; WASTE-WATER CONTAMINANTS ; SULFONAMIDES P-TSA ; FALCIPARUM-MALARIA ; NATIONAL RECONNAISSANCE ; HUMAN PHARMACEUTICALS ; AQUATIC ENVIRONMENT ; UNITED-STATES ; O-TSA ; BIODEGRADATION |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences[KZCX2-EW-206]; National Natural Science Foundation of China (NSFC)[20975089]; Doctoral Foundation of Shandong Province[BS2011SW056]; Department of Science and Technology of Yantai City of China[2010235]; Chinese Academy of Sciences |
| 原文出处 | http://www.springerlink.com/content/0923-9820/ |
| 语种 | 英语 |
| WOS记录号 | WOS:000303377300009 |
| 公开日期 | 2012-06-13 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/5504]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Weiwei,Xu, Dongxue,Niu, Zongliang,et al. Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine[J]. BIODEGRADATION,2012,23(3):431-439. |
| APA | Zhang, Weiwei,Xu, Dongxue,Niu, Zongliang,Yin, Kun,Liu, Ping,&Chen, Lingxin.(2012).Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine.BIODEGRADATION,23(3),431-439. |
| MLA | Zhang, Weiwei,et al."Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine".BIODEGRADATION 23.3(2012):431-439. |
入库方式： OAI收割
来源：烟台海岸带研究所
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